An aerobic, Gram-stain-negative, non-sporulating, flagellated and spindle-like bacterium, designated HY14T, was isolated from a pickle-processing factory wastewater sample. The isolate chemoheterotrophically grew at 4–42 °C (optimum, 35 °C) and pH 5.5–9.0 (optimum, pH 6.0–6.5). Salt was required for growth (0.5–12 % NaCl, w/v). A deep brown and water-soluble uncharacterized pigment was produced when grown in certain media. The predominant fatty acids (>5 %) included C16 : 0, C18 : 1  ω7c, 11-methyl C18 : 1  ω7c and C19 : 0 cyclo ω8c. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids, two unidentified phospholipids, two unidentified glycolipids and five unknown lipids. The major isoprenoid quinone was ubiquinone-10. Pairwise alignment based on 16S rRNA gene sequences indicated that strain HY14T had the highest sequence similarity to genera Maritimibacter (95.61–96.05 %) and Boseongicola (95.82 %). Phylogenetic analysis based on core genome illustrated that strain HY14T formed a monophyletic lineage with members of the genus Maritimibacter in the clade of the Roseobacter group in the family Rhodobacteraeceae. The core-gene average amino acid identity used to define bacterial genera by a threshold of 60–80 % was calculated to be 68.56–76.5 % between HY14T and closely related taxa. Several genomic characteristics, such as carrying two RuBisCO-mediated pathways and different osmoprotectant transport pathways, exhibited the genotypic discrepancies of strain HY14T. Based on the polyphasic taxonomic characterization, strain HY14T is considered to represent a novel species of a novel genus belonging to the family Rhodobacteraeceae, for which the name Muriiphilus fusiformis gen. nov., sp. nov. is proposed. The type strain is HY14T (=CGMCC 1.15973T=KCTC 52499T). Maritimibacter lacisalsi (Zhong et al. 2015) is considered to diverge from Maritimibacter alkaliphilus at the genus level, and should be reassigned as a novel genus, for which the name Muriicola lacisalsi gen. nov., comb. nov. is proposed.

Pseudomonas is a large genus that inhabits diverse environments due to its distinct metabolic versatility. Its applications range from environmental to industrial biotechnology. Molecular tools that allow precise and efficient genetic manipulation are required to understand and harness its full potential. Here, we report the development of a highly efficient adenine base editing system, i.e., dxABE-PS, for Pseudomonas species. The system allows A:T → G:C transition with up to 100% efficiency along a broad target spectrum because we use xCas9 3.7, which recognizes NG PAM. To enhance the dxABE-PS utility, we develop a prediction workflow for protein dysfunction using ABE, namely, DABE-CSP (dysfunction via ABE through CRISPOR-SIFT prediction). We applied DABE-CSP to inactivate several genes in Pseudomonas putida KT2440 to accumulate a nylon precursor, i.e., muconic acid from catechol with 100% yield. Moreover, we expanded the ABE to non-model Pseudomonas species by developing an nxABE system for P. chengduensisDY56−96, isolated from sediment samples from the seamount area in the West Pacific Ocean. Taken together, the establishment of the ABE systems along with DABE-CSP will fast-track research on Pseudomonas species.

In the published version of the article, the name of Muriicola gen. nov. was illegitimate, as it is a later homonym of Muriicola Kahng et al. 2010 which belongs to the family Flavobacteriaceae. Alternative names for Muriicola and Muriicola lacisalsi are proposed and the associated description sections are re-printed below: DESCRIPTION OF SINISALIBACTER GEN. NOV. Sinisalibacter ( Si. ni. sa. li. bac'ter. N.L. fem. pl. n. Sinae China; L. masc. n. sal, salis salt; N.L. masc. n. bacter a rod; N.L. masc. n. Sinisalibacter a salted rod from China).Cells are aerobic, Gram-stain-negative, non-flagellated and rod-shaped. Catalase and oxidase are positive. The predominant fatty acids (>5 %) include C 16 : 0 , C 18 : 1 ω7c, 11-methyl C 18 : 1 ω7c and C 19 : 0 cyclo ω8c. The polar lipid profile is composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipids, an unidentified phospholipid, three unidentified glycolipids and an unknown lipid. The major isoprenoid quinone is ubiquinone-10. A member of the family Rhodobacteraceae, order Rhodobacterales according to phylogenomics. The type species is Sinisalibacter lacisalsi. DESCRIPTION OF SINISALIBACTER LACISALSI COMB. NOV.Sinisalibacter lacisalsi ( la. ci. sal'si. L. masc. n. lacus lake; L. masc. adj. salsus salted; N.L. gen. n. lacisalsi of a salt lake, the habitat from which the type strain was isolated).Basonym: Maritimibacter lacisalsi Zhong et al. 2015.The following properties supplement the genus description and are basically the same as for M. lacisalsi (Zhong et al. 2015) except for some supplements and emendations as follows.No hydrolysis of CM-cellulose, DNA, pectin, skimmed milk or xylan. Negative in methyl red and Voges-Proskauer tests. Grows on Luria-Bertani agar, nutrient agar and Reasoner's 2A agar, but not on centrimide agar or MacConkey agar, all of which contain NaCl up to 20 g l −1 . In ATB 32GN tests, 2-keto-gluconate and valerate are assimilated. In API 20NE tests, positive for nitrate reduction, hydrolysis of aesculin and assimilation of adipic acid, maltose and potassium gluconate. In API ZYM tests, activity of naphthol-AS-BI-phosphohydrolase is variable. In API 50CH tests, acid is produced from ᴅarabinose, ʟ-arabinose, ᴅarabitol, cellobiose, aesculin, ᴅ-fructose, ᴅ-fucose, ʟ-fucose, ᴅ-galactose, ᴅ-glucose, glycerol, lactose, maltose, ᴅ-mannose, ᴅ-mannitol, potassium 5-ketogluconate, sucrose, salicin, ᴅ-sorbitol, ᴅ-turanose and ᴅ-xylose. Resistance to norfloxacin (10 µg), streptomycin sulphate (10 µg) and tetracycline (30 µg) is variable.

Three Gram-staining-negative, aerobic and rod-shaped strains, designated as T40-1T, T40-3T and JL-62T, were isolated from the deep-sea water in the southwest Indian ridge. For strain T40-1T, growth occurred at 15–37 °C (optimum, 28 °C), pH 6.0–9.0 (optimum, pH 7.5) and in the presence of 0.5–5.0 % NaCl (w/v; optimum, 2.0 %). Strain T40-3T could grow at 15–40 °C (optimum, 28 °C), with 0.5–11.0 % NaCl (optimum, 2.0 %, w/v) at pH 6.0–9.5 (optimum, 8.0). The temperature, pH and salinity ranges for growth of strain JL-62T were 15–40 °C (optimum, 30 °C), pH 5.5–9.0 (optimum, pH 7.5–8.0) and 0.5–9.0 % NaCl (w/v; optimum, 4.0 %). Ubiquinone-10 was the sole ubiquinone in all strains, the major fatty acids (>20 %) were summed feature 8 (C18 : 1  ω7c / C18 : 1  ω6c). The major polar lipids of strains T40-1T and T40-3T were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. Strain JL-62T contained phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and sulfoquinovosyldiacylglycerol as major polar lipids. Phylogenetic trees based on 16S rRNA gene and core-genomic sequences revealed affiliation of strains T40-1Tand T40-3T to the family Roseobacteraceae and formed two independent clades from other Roseobacteraceae genera, and those two strains had average nucleotide identities of 62.0–72.0 % to their phylogenetically related species which fell into to the genus boundary range, indicating that they represent two novel genera. While strain JL-62T represents a novel species in the genus Oricola belonging to the family Phyllobacteriaceae , which was supported by overall genomic relatedness index calculations. The DNA G+C contents of strains T40-1T, T40-3T and JL-62T were 66.5, 60.1 and 62.1 mol %, respectively. Based on the polyphasic taxonomic data, strains T40-1T (=MCCC M24557T=KCTC 82975T) and T40-3T (=MCCC 1K05135T=KCTC 82976T) are classified as representing two novel genera belonging to the family Roseobacteraceae with the names Mesobacterium pallidum gen. nov., sp. nov. and Heliomarina baculiformis gen. nov., sp. nov. are proposed, and strain JL-62T (=MCCC M24579T=KCTC 82974T) is proposed to represent a novel species within the genus Oricola with the name Oricola indica sp. nov. is proposed.