Muriiphilus fusiformis gen. nov., sp. nov., a novel non-marine bacterium belonging to the Roseobacter group, and reclassification of Maritimibacter lacisalsi (Zhong et al. 2015) as Muriicola lacisalsi gen. nov., comb. nov.

Abstract

An aerobic, Gram-stain-negative, non-sporulating, flagellated and spindle-like bacterium, designated HY14T, was isolated from a pickle-processing factory wastewater sample. The isolate chemoheterotrophically grew at 4–42 °C (optimum, 35 °C) and pH 5.5–9.0 (optimum, pH 6.0–6.5). Salt was required for growth (0.5–12 % NaCl, w/v). A deep brown and water-soluble uncharacterized pigment was produced when grown in certain media. The predominant fatty acids (>5 %) included C16 : 0, C18 : 1 ω7c, 11-methyl C18 : 1 ω7c and C19 : 0 cyclo ω8c. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids, two unidentified phospholipids, two unidentified glycolipids and five unknown lipids. The major isoprenoid quinone was ubiquinone-10. Pairwise alignment based on 16S rRNA gene sequences indicated that strain HY14T had the highest sequence similarity to genera Maritimibacter (95.61–96.05 %) and Boseongicola (95.82 %). Phylogenetic analysis based on core genome illustrated that strain HY14T formed a monophyletic lineage with members of the genus Maritimibacter in the clade of the Roseobacter group in the family Rhodobacteraeceae. The core-gene average amino acid identity used to define bacterial genera by a threshold of 60–80 % was calculated to be 68.56–76.5 % between HY14T and closely related taxa. Several genomic characteristics, such as carrying two RuBisCO-mediated pathways and different osmoprotectant transport pathways, exhibited the genotypic discrepancies of strain HY14T. Based on the polyphasic taxonomic characterization, strain HY14T is considered to represent a novel species of a novel genus belonging to the family Rhodobacteraeceae, for which the name Muriiphilus fusiformis gen. nov., sp. nov. is proposed. The type strain is HY14T (=CGMCC 1.15973T=KCTC 52499T). Maritimibacter lacisalsi (Zhong et al. 2015) is considered to diverge from Maritimibacter alkaliphilus at the genus level, and should be reassigned as a novel genus, for which the name Muriicola lacisalsi gen. nov., comb. nov. is proposed.

Maritimibacter dapengensis sp. nov., a poly-β-hydroxyalkanoates-producing bacterium isolated from sediment of the Dapeng peninsula (Guangdong, China)

“…S2) phylogenetic trees illustrated that DP4N28-5 T formed a clear clade with the type strain M. alkaliphilus DSM 100037 T . Additionally, M. lacisalsi X12M-4 T clustered with the genus Roseibacterium in both the NJ tree and the ME tree, and formed a singular lineage in the ML tree, further corroborating the reclassification of M. lacisalsi X12M-4 T [3].…”

“…lacisalsi X12M-4 T clustered with the genus Roseibacterium in both the NJ tree and the ME tree, and formed a singular lineage in the ML tree, further corroborating the reclassification of M. lacisalsi X12M-4 T [3].…”

“…Muriiphilus fusifromis HY14 T , the type strain of a novel genus Muriiphilus proposed by Wang et al . [3], which has a close relationship with M. lacisalsi X12M-4 T , was also included in the phylogenetic analysis.…”

“…The 16S rRNA gene sequences of strain DP4N28-5 T and related species were aligned using ClustalW, version 2.0 [11]. Muriiphilus fusifromis HY14 T , the type strain of a novel genus Muriiphilus proposed by Wang et al [3], which has a close relationship with M. lacisalsi X12M-4 T , was also included in the phylogenetic analysis.…”

“… M. alkaliphilus was the only species in the genus Maritimibacter , since Maritimibacter lacisalsi X12M-4 T [2] was moved from the genus Maritimibacter to a new genus and named ‘ Muriicola lacisalsi ’ in 2021 [3]. Because the new name ‘ Muriicola lacisalsi ’ has yet to be recognized as legitimate by the List of Prokaryotic Names with Standing in Nomenclature (https://lpsn.dsmz.de/species/muriicola-lacisalsi), Maritimibacter lacisalsi was chosen to represent this strain in the present study.…”