Pathological cardiac hypertrophy induced by angiotensin II (AngII) can subsequently give rise to heart failure, a leading cause of mortality. Nardosinone is a pharmacologically active compound extracted from the roots of Nardostachys chinensis, a well-known traditional Chinese medicine. In order to investigate the effects of nardosinone on AngII-induced cardiac cell hypertrophy and the related mechanisms, the myoblast cell line H9c2, derived from embryonic rat heart, was treated with nardosinone (25, 50, 100, and 200 μmol/L) or AngII (1 μmol/L). Then cell surface area and mRNA expression of classical markers of hypertrophy were detected. The related protein levels in PI3K/Akt/mTOR and MEK/ERK signaling pathways were examined by Western blotting. It was found that pretreatment with nardosinone could significantly inhibit the enlargement of cell surface area induced by AngII. The mRNA expression of ANP, BNP and β-MHC was obviously elevated in AngII-treated H9c2 cells, which could be effectively blocked by nardosinone at the concentration of 100 μmol/L. Further study revealed that the protective effects of nardosinone might be mediated by repressing the phosphorylation of related proteins in PI3K/Akt and MEK/ERK signaling pathways. It was suggested that the inhibitory effect of nardosinone on Ang II-induced hypertrophy in H9c2 cells might be mediated by targeting PI3K/Akt and MEK/ERK signaling pathways.
The purpose of this study was to develop a novel method to precisely measure the arc length of curving skeleton. We developed a formula by using differential equation and the arc length of bending bones can be calculated according to the chord length and height. With this method, we measured the coronal arc length of parietal bones in fetus. Our results showed that the arc length calculated using the formula was not significantly different from those obtained by manual measurements (P>0.05). We concluded that this method was simple, precise and can be used to measure the ossification length of any fetus parietal bones with less than 180o curvature or the arc length of bones in other parts of humans.
According to the wavelet-neural network algorithm of relay protection device has the characteristics of requirement of high sampling frequency and large amount of data calculation, we use FPGA to realize the algorithm in hardware. A system model is built and converted into VHDL code through the system-level digital signal processing development tool named DSP Builder from Altera Company. We verified the correctness and feasibility of the method by the timing simulation diagram get from Modelsim SE software. This paper implemented protection method of the wavelet transform and neural network with hardware, and completed a series of research work in the field of transformer differential protection.
A smart meter was designed with power line communication (PLC) function. We designed the optimized power carrier module by adding a switching circuit that controlled by a microcontroller in coupling circuit on the basis of common design. Improved its communication efficiency and reduced energy consumption. Energy metering module used C8051F360 microcontroller as the main control chip and used high accurate metering chip ATT7022B to collect electronic signal. We designed a multifunctional and easy-to-use intelligent man-machine interface by using Beijing DWIN Company’s industrial serial LCD touch screen.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.