Bombyx mori cocoon has a multi-layer structure that provides optimal protection for silkworm pupa. Research on the mechanical properties of the multi-layer structure revealed structure-property relationships of the cocoon. Here, we investigated the protein components of the B. mori cocoon in terms of its multi-layer structure. Liquid chromatography-tandem mass spectrometry identified 286 proteins from the multiple cocoon layers. In addition to fibroins and sericins, we identified abundant protease inhibitors, seroins and proteins of unknown function. By comparing protein abundance across layers, we found that the outermost layer contained more sericin1 and protease inhibitors and the innermost layer had more seroin1. As many as 36 protease inhibitors were identified in cocoons, showing efficient inhibitory activities against a fungal protease. Thus, we propose that more abundant protease inhibitors in the outer cocoon layers may provide better protection for the cocoon. This study increases our understanding of the multi-layer mechanism of cocoons, and helps clarify the biological characteristics of cocoons. The data have been deposited to the ProteomeXchange with identifier PXD001469.
This study investigated the concept of individual differences (IDs) in the use of metacognitive strategies (planning, problem-solving, monitoring, and evaluating) and its relationship with task demand and learner performance within Kormos’ Bilingual Speech Production Model from the lens of Chinese English-as-foreign-language (EFL) learners in the context of integrated L2 speaking assessment. To measure metacognitive strategies, we administered an inventory on 134 Chinese EFL learners after they completed four integrated L2 speaking assessment tasks. Descriptive analysis and multiple linear regression were adopted for data analysis, and results show that: (a) IDs displayed variance in Chinese EFL learners’ metacognitive strategy use; (b) among the four metacognitive strategies under investigation, problem-solving was reported to be used the most frequently in sharp contrast to monitoring, which had the lowest frequency; (c) metacognitive strategies worked interactively, responding to task demands involved in the four integrated L2 speaking assessment tasks; and (d) Chinese EFL learners’ use of metacognitive strategies, in individual and interactive working modes, had no relationship with their speaking performance. These results are expected to present some insights into the role of IDs in metacognitive strategy use during L2 speech production under assessment conditions, which will add robust evidence to the existing literature on L2 speaking, in particular on metacognitive strategy use in L2 speaking assessment. In the meantime, the findings will provide some empirical validation support for Kormos’ model, which will further provide some implications for L2 speaking instruction and L2 assessment.
Understanding the intricate relationships among strategic competence, tasks and performance is an issue of perennial interest in the assessment of foreign/second languages especially in integrated speaking assessment, a field that is under-researched. Against this background, we investigated such complex relationships in the context of integrated speaking assessment of English as foreign language (EFL) learners, hoping to provide additional empirical evidence to address the problem. In the investigation, strategic competence was defined as metacognitive strategy use and was measured via an inventory administered on 120 Chinese university EFL students; task characteristics were conceptualised as task complexity and were measured on a self-rating scale by the students and five EFL teachers; and the students’ speaking performance was indicated by their scores on four integrated speaking assessment tasks. Data analysis through a hierarchy linear modelling approach led to two primary findings: Monitoring, one form of strategic competence, moderated the effect of task complexity on performance; strategic competence had no substantial effects on performance which had an inverse relationship with task complexity. These findings will add validity evidence for the foreign language speaking assessment literature and provide implications for speaking instruction and test development.
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Introduction Disease‐causing pathogens in food are transmitted far and wide through current interconnected global food‐chains and the morbidity of food‐borne diseases is increasing dramatically. It is quite important for every county to share information and experience with each other in order to optimize monitoring system of food safety. Objectives To help people from other countries understand more about Chinese food safety information by introducing the application of polymerase chain reaction technique in the detection of food‐borne pathogens and the food safety supervision system. Methods Official information and articles in Chinese Journal Full‐Text Database and other academic databases have been searched and analyzed. Results The types of food‐borne pathogens detected by polymerase chain reaction have increased dramatically and more applications of quantitative polymerase chain reaction in recent years have been observed. The implementation of a new food safety law has been strengthened and the supervision system of food safety optimized. Conclusion Techniques for improving the monitoring of food safety and the implementation of the new food safety law will improve the food safety situation in China significantly. This paper will be very helpful for people to understand more about the current Chinese food safety system.
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