Comparative Proteome Analysis of Multi-Layer Cocoon of the Silkworm, Bombyx mori

Zhao, Ping; Wang, Dandan; Guo, Pengchao; Guo, Xiaomeng; Song, Qianru; Zhang, Weiwei; Xia, Qingyou

doi:10.1371/journal.pone.0123403

Cited by 37 publications

(64 citation statements)

References 36 publications

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“…3, designated as spot 5Ј), but we suspect that this spot was some other protein(s) as Fhxh4/ Fhxh5 transcripts were not detected in normal PSGs (see the description later). Fhxh4/Fhxh5 proteins are abundant in the cocoon of normal individuals (37) and are predicted to be major silk proteins similar to sericins.…”

Section: Identification Of Novel Antpmentioning

confidence: 99%

A Hox Gene, Antennapedia, Regulates Expression of Multiple Major Silk Protein Genes in the Silkworm Bombyx mori

Tsubota

Tomita

Uchino

et al. 2016

Journal of Biological Chemistry

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Hox genes play a pivotal role in the determination of anteroposterior axis specificity during bilaterian animal development. They do so by acting as a master control and regulating the expression of genes important for development. Recently, however, we showed that Hox genes can also function in terminally differentiated tissue of the lepidopteran Bombyx mori. In this species, Antennapedia (Antp) regulates expression of sericin-1, a major silk protein gene, in the silk gland. Here, we investigated whether Antp can regulate expression of multiple genes in this tissue. By means of proteomic, RT-PCR, and in situ hybridization analyses, we demonstrate that misexpression of Antp in the posterior silk gland induced ectopic expression of major silk protein genes such as sericin-3, fhxh4, and fhxh5. These genes are normally expressed specifically in the middle silk gland as is Antp. Therefore, the evidence strongly suggests that Antp activates these silk protein genes in the middle silk gland. The putative sericin-1 activator complex (middle silk gland-intermoltspecific complex) can bind to the upstream regions of these genes, suggesting that Antp directly activates their expression. We also found that the pattern of gene expression was well conserved between B. mori and the wild species Bombyx mandarina, indicating that the gene regulation mechanism identified here is an evolutionarily conserved mechanism and not an artifact of the domestication of B. mori. We suggest that Hox genes have a role as a master control in terminally differentiated tissues, possibly acting as a primary regulator for a range of physiological processes.

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Section: Identification Of Novel Antpmentioning

confidence: 99%

A Hox Gene, Antennapedia, Regulates Expression of Multiple Major Silk Protein Genes in the Silkworm Bombyx mori

Tsubota

Tomita

Uchino

et al. 2016

Journal of Biological Chemistry

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“…The composition of silk fibroin is almost consistent from the innermost to the outermost layers of the silkworm cocoon [101]. Silk sericin is considered as a bonding protein among hydrophilic silk fibers.…”

Section: Composition Of Silk Matmentioning

confidence: 94%

“…The sericin in the innermost layer is more or less 15%, and in the outermost layer, it is 25% [102]. Seroin is considered to be an anti-septic protein, and its content decreases from the inner to the outer layers [101]. The amount of various protease inhibitors is also different according to the layer [103].…”

Section: Composition Of Silk Matmentioning

confidence: 99%

“…In the case of the silk mat, natural sericin will be released in a fragmented form. In addition, the content of sericin is different from the layers of the cocoon [101]. Considering dose-dependent TNF-α induction by denatured sericin [55], the sericin content in the silk mat should be small.…”

Section: In Vitro Properties Of Silk Matmentioning

confidence: 99%

“…As the content of sericin is increased from the inner to the outer layers, the silk mat from the inner layer may be better than that from the outer layer. There are other bonding proteins to sericin, such as seroin and protease inhibitors [101]. Although few studies have been conducted on these proteins, they may induce TNF-α-like sericin.…”

Section: In Vitro Properties Of Silk Matmentioning

confidence: 99%

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Silk Protein-Based Membrane for Guided Bone Regeneration

Kwon

Seok

2018

Applied Sciences

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Silk derived from the silkworm is known for its excellent biological and mechanical properties. It has been used in various fields as a biomaterial, especially in bone tissue engineering scaffolding. Recently, silk protein-based biomaterial has been used as a barrier membrane scaffolding for guided bone regeneration (GBR). GBR promotes bone regeneration in bone defect areas using special barrier membranes. GBR membranes should have biocompatibility, biodegradability, cell occlusion, the mechanical properties of space-making, and easy clinical handling. Silk-based biomaterial has excellent biologic and mechanical properties that make it a good candidate to be used as GBR membranes. Recently, various forms of silk protein-based membranes have been introduced, demonstrating excellent bone regeneration ability, including osteogenic cell proliferation and osteogenic gene expression, and promoting new bone regeneration in vivo. In this article, we introduced the characteristics of silk protein as bone tissue engineering scaffolding and the recent application of such silk material as a GBR membrane. We also suggested future studies exploring additional uses of silk-based materials as GBR membranes.

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Role of sericin 1 in the immune system of silkworms revealed by transcriptomic and proteomic analyses after gene knockout

Zhao

et al. 2021

FEBS Open Bio

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The domestic silkworm is a type of lepidopteran insect that feeds on mulberry leaves and has high economic value because of its ability to spin cocoons. Sericin 1 is an important component of silkworm cocoons, accounting for approximately 25% of the material. In this study, CRISPR/Cas9‐mediated gene editing was successfully used to destroy the sericin 1 gene, and homozygous mutants were obtained after continuous screening. Homozygous mutation resulted in premature termination of the translation of sericin 1 protein at 323 amino acids. Comparative transcriptomic and proteomic analyses of middle silk gland cells from wild‐type individuals and mutants were performed on the fourth day of the fifth instar, and the results suggest that sericin 1 plays an important role in the cellular immune system. In addition, the results suggest that sericin 1 has a synergistic effect with some protease inhibitors and that the secretion of these proteins is strictly regulated. These results will provide new insights into the function and expression pattern of sericin 1 and the mechanism of silk secretion.

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Comparative Proteome Analysis of Multi-Layer Cocoon of the Silkworm, Bombyx mori

Cited by 37 publications

References 36 publications

A Hox Gene, Antennapedia, Regulates Expression of Multiple Major Silk Protein Genes in the Silkworm Bombyx mori

A Hox Gene, Antennapedia, Regulates Expression of Multiple Major Silk Protein Genes in the Silkworm Bombyx mori

Silk Protein-Based Membrane for Guided Bone Regeneration

Role of sericin 1 in the immune system of silkworms revealed by transcriptomic and proteomic analyses after gene knockout

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