Curcumin (diferuloylmethane) is an active component of the spice turmeric and has a diversity of antitumor activities. In this study, we found that curcumin can inhibit cancer cell invasion and metastasis through activation of the tumor suppressor DnaJ-like heat shock protein 40 (HLJ1). Human lung adenocarcinoma cells (CL1-5) treated with curcumin (1-20 Mmol/L) showed a concentration-dependent reduction in cell migration, invasion, and metastatic ability, and this was associated with increased HLJ1 expression. Knockdown of HLJ1 expression by siRNA was able to reverse the curcumininduced anti-invasive and antimetastasis effects in vitro and in vivo. The HLJ1 promoter and enhancer in a luciferase reporter assay revealed that curcumin transcriptionally upregulates HLJ1 expression through an activator protein (AP-1) site within the HLJ1 enhancer. JunD, one of the AP-1 components, was significantly up-regulated by curcumin (1-20 Mmol/L) in a concentration-and time-dependent manner. Knockdown of JunD expression could partially reduce the curcumin-induced HLJ1 activation and diminish the anti-invasive effect of curcumin, indicating that JunD would seem to be involved in curcumin-induced HLJ1 expression. Curcumin was able to induce c-Jun NH 2 -kinase (JNK) phosphorylation, whereas the JNK inhibitor (SP-600125) could attenuate curcumin-induced JunD and HLJ1 expression. Activation of HLJ1 by curcumin further leads to up-regulation of E-cadherin and a suppression of cancer cell invasion. Our results show that curcumin induces HLJ1, through activation of the JNK/JunD pathway, and inhibits lung cancer cell invasion and metastasis by modulating E-cadherin expression. This is a novel mechanism and supports the application of curcumin in anti-cancer metastasis therapy. [Cancer Res 2008;68(18):7428-38]

Despite good initial responses, drug resistance and disease recurrence remain major issues for lung adenocarcinoma patients with epidermal growth factor receptor (EGFR) mutations taking EGFR-tyrosine kinase inhibitors (TKI). To discover new strategies to overcome this issue, we investigated 40 essential oils from plants indigenous to Taiwan as alternative treatments for a wide range of illnesses. Here, we found that hinokitiol, a natural monoterpenoid from the heartwood of Calocedrus formosana, exhibited potent anticancer effects. In this study, we demonstrated that hinokitiol inhibited the proliferation and colony formation ability of lung adenocarcinoma cells as well as the EGFR-TKI-resistant lines PC9-IR and H1975. Transcriptomic analysis and pathway prediction algorithms indicated that the main implicated pathways included DNA damage, autophagy, and cell cycle. Further investigations confirmed that in lung cancer cells, hinokitiol inhibited cell proliferation by inducing the p53-independent DNA damage response, autophagy (not apoptosis), S-phase cell cycle arrest, and senescence. Furthermore, hinokitiol inhibited the growth of xenograft tumors in association with DNA damage and autophagy but exhibited fewer effects on lung stromal fibroblasts. In summary, we demonstrated novel mechanisms by which hinokitiol, an essential oil extract, acted as a promising anticancer agent to overcome EGFR-TKI resistance in lung cancer cells via inducing DNA damage, autophagy, cell cycle arrest, and senescence in vitro and in vivo.

The results suggest that serum miR-125b levels are associated with VC severity and serve as a novel predictive marker for the risk of uremia-associated calcification progression.

The current study illustrated the time variance of turning points in the relationship between carbon emissions and income to resolve heated debate on the different responsibility to climate change with 1950–2010 data of five development diversity countries—three developed countries (Germany, Italy, and Japan) and one developing country (India) and one newly industrialized economy (Taiwan). The article also examines the impact of the crisis on emission. The time-varying patterns in the turning points on environmental Kuznets curves (EKCs) were observed by a rolling regression technique with 1950–2010 data regarding the per capita CO2 emissions caused by fossil fuel combustion and the incomes of the countries. Several empirical findings were revealed from this analysis. Per capita CO2 emissions commonly decreased with varying magnitudes in the five countries over time. The EKC hypothesis regarding the CO2 emissions is affirmed again in this study. The announcement effects associated with the Kyoto Protocol was evidenced. As indicated by the occurring GDP of the turning point, there is a strong reduction trend in the income level of the turning points right before the years of Kyoto Protocol; and this decreasing trend nearly ended as the Kyoto protocol approached its end, except in Germany, where the occurring income of the turning points continued to have a decreasing trend. Although the global financial crisis had its effects in the world, the impacts on carbon dioxide emissions vary across countries.

No abstract

This study investigated the willingness of residents to pay for ecosystem services in a hillside forest in the Lanyang River Basin, which is among the most vulnerable watersheds in Taiwan. The economic value of provisioning, regulating, cultural, and supporting ecosystem services was evaluated. The Contingent Valuation Method (CVM) was applied for economic analysis of public welfare. The determinants of the economic values were identified. A total of 444 respondents completed the questionnaire. The results revealed that the four ecosystem services had high economic value, indicating that conserving hillside forests can ensure the welfare of nearby residents. The findings of this study can serve as reference for regional land planning and social and economic system development policies. In addition, this study addressed policy implementation from the perspective of ecological economics to contribute to an improved Anthropocene.

To clarify the effects of generalized capitals and energy footprint on aggregate incomes and total carbon dioxide emissions, a cross-country panel analysis is applied in the present study. The generalized capitals included in this study are human capital, manufacture capital, natural capitals (as rents of fossil fuels, forest, and minerals). The energy footprint is represented by the primary energy consumption to index the overall domestic energy use. A Cobb–Douglas production function is used to empirically study on a panel of 21 European Union countries. Annual data of rents of natural capitals are used to represent the economic value of natural capitals that flows to the economy. The following are the main findings of this study: (1) Employing human and manufactural capital makes contributions to income growth and carbon reduction. This study’s evidence guides to clarify the misunderstanding of capital and capitalism. Innovations through well-developed and well-managed human and manufactured capital can help sustain income and reduce carbon dioxide emissions. (2) Energy footprint is the vital determinant to total carbon dioxide emissions and hence the most important part of climate policy. (3) The value currently commeasured by monetary terms and compiled by the World Bank is evidenced, not persistently contributed to the income, rather contributed to total carbon dioxide emissions, for the sake of the energy-intensive attributes in the resource-extracting industry. The natural capitals represented by the rent of extracting endowed natural resources can only represent part of the value of natural capitals to human beings. The virtue values of natural capitals in terms of amenity and life supporting are inevitable, but intangible and hence incommensurable. This value is still ignored and unable to enter the contemporary gate of monetary national accounting system.

The purpose of energy sustainability policy is to support both economic growth and environmental quality. With climate change accelerating, economies must reduce carbon emissions. Low-carbon economics can balance the oft-contradictory policy aims of income growth and carbon reduction. Carbon pricing and renewable substitutes can pave the way. This analysis probes the dynamics of the adjustments toward the ideals of low-carbon economics through Granger causality testing of total carbon emissions, income, nonrenewable energy consumption, and renewable power. Cointegration regressions and a panel data vector error correction model are used to demonstrate the aforementioned variables’ long-term balance and short-term adjustment, respectively. Two panels of countries, namely 18 European Union and 32 Organization of Economic Co-operation and Development countries, are investigated with 1990–2021 data. Determinants for the success of low-carbon development and the implications of border regulations and taxation of carbon footprint are also discussed. Economic competitiveness, as well as increases in commodity prices, would initially emerge as interferences and then induce carbon reduction and accelerate the adoption and development of green technology.