Sir: The accurate determination of serum protein-bound carbohydrate by the anthrone method (S) is hindered by the simultaneous release of tryptophan from the protein (5-7). Dissatisfaction with existing modifications (1, 2, 6, 7) prompted further study of the behavior of carbohydrate and tryptophan with anthrone.Confirming the work of Tuller and Keiding (7) for a given concentration of carbohydrate, all the absorption curves converged at 585 µ, despite varying concentrations of tryptophan. This observation offers an effective solution to the tryptophan interference problem.This communication establishes the validity of a modified anthrone method, which is compared with existing modifications.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.