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Extended cultivation with multiple removal of BC pellicles is proposed herein as a new biosynthetic process for bacterial cellulose (BC). This method enhances the BC surface area by 5–11 times per unit volume of the growth medium, improving the economic efficiency of biosynthesis. The resultant BC gel-films were thin, transparent, and congruent. The degree of polymerization (DP) and elastic modulus (EM) depended on the number of BC pellicle removals, vessel shape, and volume. The quality of BC from removals II–III to VII was better than from removal I. The process scale-up of 1:40 by volume increased DP by 1.5 times and EM by 5 times. A fact was established that the symbiotic Medusomyces gisevii Sa-12 was adaptable to exhausted growth medium: the medium was able to biosynthesize BC for 60 days, while glucose ran low at 24 days. On extended cultivation, DP and EM were found to decline by 39–64% and 57–65%, respectively. The BC gel-films obtained upon removals I–VI were successfully trialed in experimental tension-free hernioplasty.

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Background. Enteroatmospheric fistulas (EAF) that occur during the use of the “open abdomen” surgical tactics are a complex surgical pathology with a high mortality rate.The aim. To assess the effectiveness of treatment of various forms of enteroatmospheric fistulas in patients with postoperative peritonitis using vacuum aspiration technology.Methods. We assessed the results of the surgical treatment of 46 patients with EAF in the late course of postoperative peritonitis (PP). Three clinical and morphological groups were distinguished: group 1 (n = 24) – EAF in small wounds of the anterior abdominal wall; group 2 (n = 15) – EAF opening into limited cavities; group 3 (n = 7) – EAF opening into laparostoma wounds. In group 1, a fistula was formed using continuous aspiration devices or VAC systems. In group 2, we used continuous aspiration of intestinal contents from the cavity. In group 3, laparostoma was treated using vacuum devices with isolation of the intestinal fistula and simulation of a floating enterostoma.Results. Group 3 of patients with EAF was characterized by a high flow rate (1224.2 ± 210.3 ml), duration of treatment (87.3 ± 12.5 day), extensive laparostoma (335.4 ± 14.3 сm2), high mortality rate (57.1 %). The best results of treatment were obtained in groups 1 and 2. The flow rate was 675.8 ± 154.3 and 541.3 ± 114.1 ml, the duration of treatment was 2 or 3 times less (37.7 ± 6.1 and 26.4 ± 5.2 days), the mortality rate was 8.3 % and 6.7 % respectively.Conclusion. EAF that occur when using the “open abdomen” surgical tactics due to the impossibility of their isolation in extensive wounds of the anterior abdominal wall are complicated clinical and morphological forms. For their treatment, it is advisable to use VAC systems, aimed at the treatment of both the anterior abdominal wall wound itself and the intestinal fistula opening into it for its gradual extraterritorialization by modeling a floating enterostoma in a vacuum device.

A new method for the treatment of chronic pancreatitis by introducing into arterial bed of pancreas or a regional arterial bed pancreas suspension of hydrocortisone. Objective: To provide local intensification anti-inflammatory effects, reducing the risk of systemic side effects. Material and methods: Endovascular treatment of 30 patients (19 males (63,3%) and 11 women (36.7%), the average age of 50.7 years. Organ (gastroduodenal artery) or regional (celiac trunk) infusion of a suspension of hydrocortisone. Indications e ineffectiveness of conservative therapy, recurrent of pain.Clinical evaluation of the dynamics of abdominal pain during the infusion, within 1 month and 6 months. A score of pain, where 0-his absence, the maximum intensity of 5.Studied indices of tissue blood flow in the stomach by a method rheoarteriogastrography at baseline, at the middle and at the end of long arterial infusion suspension of hydrocortisone in pool celiac trunk, n = 15.We studied the performance of the specific density of the pancreatic tissue during the treatment. Results: Severe pain at the time of admission to the surgical hospital. After endovascular treatment of pain intensity reduced to 0,34 AE 0,19 points.Flow condition in the stomach without ischemic changes during and after treatment.According to the CT study noted a decrease in the size of the pancreas, the restoration of the specific density of the parenchyma to normal values. Conclusions: Endovascular treatment method allows HP to stop inflammation, affect tissue fibrosis and the loss of its basic functions.

A new method for the treatment of chronic pancreatitis by introducing into arterial bed of pancreas or a regional arterial bed pancreas suspension of hydrocortisone. Objective: To provide local intensification anti-inflammatory effects, reducing the risk of systemic side effects. Material and methods: Endovascular treatment of 30 patients (19 males (63,3%) and 11 women (36.7%), the average age of 50.7 years. Organ (gastroduodenal artery) or regional (celiac trunk) infusion of a suspension of hydrocortisone. Indications e ineffectiveness of conservative therapy, recurrent of pain.Clinical evaluation of the dynamics of abdominal pain during the infusion, within 1 month and 6 months. A score of pain, where 0-his absence, the maximum intensity of 5.Studied indices of tissue blood flow in the stomach by a method rheoarteriogastrography at baseline, at the middle and at the end of long arterial infusion suspension of hydrocortisone in pool celiac trunk, n = 15.We studied the performance of the specific density of the pancreatic tissue during the treatment. Results: Severe pain at the time of admission to the surgical hospital. After endovascular treatment of pain intensity reduced to 0,34 AE 0,19 points.Flow condition in the stomach without ischemic changes during and after treatment.According to the CT study noted a decrease in the size of the pancreas, the restoration of the specific density of the parenchyma to normal values. Conclusions: Endovascular treatment method allows HP to stop inflammation, affect tissue fibrosis and the loss of its basic functions.

The article provides up-to-date statistics on the prevalence of purulent pyelonephritis in Russia, shows the pathogenesis of purulent pyelonephritis in terms of circulatory disorders in the kidney for example 23 patients operated personally by the author in Urology conditions of the Altai Krai hospital on his own technique in the period 2016 by 2017. Morphological studies were carried out in the Department of pathologic Anatomy. Assess the morphological distortion in the kidney with significant changes in the mikrocirkuljatornom vein during the operation of the apostem zone, perifokalnogo zone of inflammation around 1 cm from the pockets of destruction and of apparently healthy sections of kidney the same kidney parenchyma jelektronnomikroskopicheskim method. Additionally investigated plots tissues of the kidneys removed due to have resulted them irreversible destructive changes. Irreversible development of acute purulent pyelonephritis associated with renal tissue ischemia and to achieve good results in the treatment of patients, appropriate action must be taken to restore the lost blood flow.

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