BackgroundApolipoprotein E (APOE) polymorphism is associated with lipid levels. Some studies have reported that blood lipid response to diet or obesity varies depending on APOE genotypes. The aim of this study was to assess the effect of APOE genotypes, the intake of saturated fatty acids (SFA), and obesity on serum lipid levels in Lithuanian adult population.Methodology/Principal FindingsA cross-sectional health survey was carried out in five municipalities of Lithuania. The random sample was obtained from lists of 25–64 year-old inhabitants registered at primary health care centres. The data from 996 subjects (416 men and 580 women) were analysed in this study. Two single-nucleotide polymorphisms (rs429358 and rs7412) were assessed using a real-time polymerase chain reaction. 24-hour recall and food frequency questionnaire were used for evaluation of dietary habits. Serum lipids were determined using enzymatic methods.Men and women with the APOE2 genotype had the lowest level of total cholesterol (TC) (p = 0.002 for men, and p = 0.02 for women) and low-density lipoprotein cholesterol (LDL-C) (p<0.001). Multivariate linear regression analysis showed that age, genotype APOE2, SFA intake, and body mass index (BMI) were significant determinants of TC and LDL-C level (with p values ranging from 0.043 to 0.001). Our data did not reveal any statistically significant interactions between APOE genotype and SFA intake or between APOE genotype and BMI regarding TC and LDL-C level (all p>0.05). However, the predictive power of the regression model for LDL-C improved when gene-BMI interaction and gene-BMI interaction plus gene-nutrient interaction were added (p = 0.04 and p = 0.032 for R2 change, respectively).Conclusions/Significance APOE genotypes, SFA intake, and obesity were found to be associated with blood lipid levels in Lithuanian adult population. Analysis of gene-diet and gene-obesity interactions did not confirm that the effects of diet and obesity on TC and LDL-C level significantly depended on APOE genotype.

The aim of the study was to assess the risk profile for noncommunicable diseases in Lithuanian rural population. Material and methods. Within CINDI program, the risk factor survey was carried out in five rural regions of Lithuania in random sample of population aged 25–64 years in 2007. The risk factors were defined according to the WHO criteria. A total of 1739 participants were enrolled in the study. Results. The prevalence of hypertension was considerably higher among men than among women (60.3% and 44.6%, respectively). Hypercholesterolemia was identified in half of examined persons with no gender difference. The proportion of obese female persons was greater than male. Metabolic syndrome was diagnosed in 15.1% of men and in 21.5% of women. The significant gender difference was observed in the prevalence of regular smoking (47.5% in men and 18.1% in women) as well as risky alcohol consumption (26.7% of men and 3.1% of women). The majority of rural population was lacking leisure-time physical activity. The prevalence of hypertension, hypercholesterolemia, obesity, and metabolic syndrome was increasing with age in both men and women. Regular smoking and risky alcohol consumption were more common among younger than older population. Leisure-time physical activity tended to increase with age. Conclusions. The prevalence of risk factors for noncommunicable diseases in Lithuanian rural population is high. The obtained data prove that comprehensive and intersectorial preventive actions aimed at the reduction in the risk of noncommunicable diseases are urgently needed in Lithuania.

BackgroundSince regaining of independence in 1990, Lithuania has been undergoing substantial political, economic, and social changes that affected the nutrition habits of population. Dietary changes might have impact on the trends of dietary related risk factors of chronic diseases. The aim of the study was to compare trends in diet and lipid profile of Lithuanian rural population aged 25-64 during two decades of transition period (1987-2007).MethodsFour cross-sectional surveys were conducted within the framework of the Countrywide Integrated Noncommunicable Diseases Intervention Programme in five regions of Lithuania in 1987, 1993, 1999, and 2007. For each survey, a stratified independent random sample was drawn from the lists of the inhabitants aged 25-64 years registered at the primary health care centres. Altogether 3127 men and 3857 women participated in the surveys. 24-hour recall was used for evaluation of dietary habits. Serum lipids were determined using enzymatic methods. Predicted changes of serum cholesterol were calculated by Keys equation.ResultsThe percentage of energy from saturated fatty acids has decreased from 18.0 to 15.1 among men and from 17.6 to 14.8 among women over the period of 20 years. The average share of polyunsaturated fatty acids in total energy intake increased from 5.3% to 7.1% among men and from 4.9% to 7.3% among women. The mean intake of cholesterol declined among women. Favourable trends in fatty acids composition were caused by increased use of vegetable oil for cooking and replacement of butter spread with margarine. Since 1987, the mean value of total cholesterol has decreased by 0.6 mmol/l. Total dietary effect accounts for a 0.26 mmol/l (43.3%) decline in serum cholesterol among men and 0.31 mmol/l (50.8%) decline among women.ConclusionsImprovement in the quality of fat intake was observed in Lithuanian rural population over two decades of transition period. Positive changes in diet, mainly reduction in saturated fatty acids intake, contributed to decline in serum cholesterol level. Strengthening of favourable trends in nutrition habits in Lithuanian population should be one of the most important strategies of cardiovascular diseases prevention.

The aim of the study was to evaluate the dietary intake of a Lithuanian rural population and to assess the relationship between diet and dyslipidemias. Material and Methods. A cross-sectional health survey was carried out in 5 municipalities of Lithuania in 2007. The random sample was obtained from lists of 25- to 64-year-old inhabitants registered at primary health care centers (n=1739). The food frequency questionnaire and 24-hour recall was used for the evaluation of nutrition habits. The levels of serum lipids were determined using enzymatic methods. Factor analysis was employed in order to reduce the number of food items. The scores of food factors were used in linear regression analysis of associations between nutrition and serum lipid levels. Results. The diet of the study population was very high in fat, especially in saturated fatty acids. The content of cholesterol in the diet of men was higher than recommended. The main sources of fat and protein were meat, milk, and their products. Cereals were the main source of carbohydrates. The major proportion of monosaccharide and disaccharides was derived from confectionary and fruits. The mean serum total cholesterol level was 5.60 mmol/L in men and 5.51 mmol/L in women. Conclusions. Healthier food patterns were associated with the lower levels of total, low-density lipoprotein cholesterol, and triglyceride.