Pseudomonas aeruginosa is an aerobic, motile, gram negative rod that belongs to the family, pseudomonadaceae 2. Its general resistance is due to a combination of factors 3 .Regional variations in the antibiotic resistance exist for different organisms, including P. aeruginosa and this may be related to the difference in the antibiotic prescribing habits. So, we aimed in the present study, to determine the status of antimicrobial resistance to antipseudomonadal agents and the magnitude of the multidrug resistance in these organisms.MATERIALS AND METHODS: This study was conducted during 1 st January 2013 to 30 th September 2013. During this period total of 5877 samples were tested, out of 5877 samples, 1693 samples showed growth on culture and out of 1693 samples, 152 Pseudomonas aeruginosa were isolated. Identification & sensitivity of all isolates were done by BD Phoenix TM Automated Microbiological System. The antibiotics which were included in the panel were ciprofloxacin, levofloxacin, gentamicin, amikacin, tobramycin, aztreonam, ceftazidime, cefepime, piperacillin, piperacillin/tazobactam, ticarcillin/tazobactam, imipenem, meropenem and colistinaccording to CLSIs guidelines.RESULT: In the present study, the highest numbers of Pseudomonas infections was found in pus followed by urine and Endotracheal secretion. Pseudomonas aeruginosa isolated from various samples were resistant to aztreonam, ciprofloxacin followed by levofloxacin, ceftazidime, cefepime, amikacin, imipenem & colistin.CONCLUSION: To prevent the spread of the resistant bacteria, it is critically important to have strict antibiotic policies wherein surveillance programmes for multidrug resistant organisms and infection control procedures need to be implemented.
Calcification is frequently seen histopathological and radiological finding in renal cell carcinoma (RCC). Occurrence of metaplastic bone is rare feature and majority are reported in clear cell renal cell carcinoma. We report a case of papillary renal cell carcinoma with extensive osseous metaplasia.
Aim: Tuberculosis which is an infectious disease caused by M. tuberculosis causing pulmonary and extra pulmonary tuberculosis in clinical suspects in Indore and region around central state of India by IGRA methods. In India, TB which is declared to be 'notifiable disease of the nation' by the RNTCP since 2012. We wanted to analyze present state of existence of the same in our city Indore and region around. We wanted to study the present strata of IGRA detected in population of Indore in MP correlating with the Mycobacterium isolates in our laboratory, though the protocol of DOTS have been followed in the country. Experimental design:In present study, we tested clinical suspects using microbiology biology cultivation method and ELISA method for IGRA in Indore for Mycobacterium tuberculosis in the clinical suspects in our Indore lab, MP, India. Place and duration:The study was one in Central lab-Oncquest India Ltd. in Indore between period 2012 to 2014.Methodologies: The present study included 135 patients, including 49 male, and 86 female patients. We used developed TB-TMA method (Oncquest, Ltd.) to detect infection of clinical suspects and utilized culture susceptibility test to detect drug resistance in infecting Mycobacterium causing tuberculosis, tested at Central lab-Oncquest ltd. in India using microbiological methods. The method of drug resistance in Mycobacteria was performed using microbiology methods of drug resistance as described by Songara P, 2015. Results:We found 53% samples to be positive from male group compared to 29% from female group of patients. We could isolate Mycobacterium sp from various clinical suspects using basic microbiology and cultivation methods. Were found 41.6% Mycobacterium to be sensitive to sensitive to INH 36.65 to RIF, 23.3 to PYRA, 305 to ETHM, 25% to STREPTO isolated from various samples from clinical suspects. Conclusion:We were able to detect M. tuberculosis and determine their drug resistance in Mycobacterium method by MDR sure method.
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