Antibiotic Resistance Pattern in Pseudomonas Aeruginosa Species Isolated at Indore (M.P.)

Abstract

Pseudomonas aeruginosa is an aerobic, motile, gram negative rod that belongs to the family, pseudomonadaceae 2. Its general resistance is due to a combination of factors 3 .Regional variations in the antibiotic resistance exist for different organisms, including P. aeruginosa and this may be related to the difference in the antibiotic prescribing habits. So, we aimed in the present study, to determine the status of antimicrobial resistance to antipseudomonadal agents and the magnitude of the multidrug resistance in these organisms.MATERIALS AND METHODS: This study was conducted during 1 st January 2013 to 30 th September 2013. During this period total of 5877 samples were tested, out of 5877 samples, 1693 samples showed growth on culture and out of 1693 samples, 152 Pseudomonas aeruginosa were isolated. Identification & sensitivity of all isolates were done by BD Phoenix TM Automated Microbiological System. The antibiotics which were included in the panel were ciprofloxacin, levofloxacin, gentamicin, amikacin, tobramycin, aztreonam, ceftazidime, cefepime, piperacillin, piperacillin/tazobactam, ticarcillin/tazobactam, imipenem, meropenem and colistinaccording to CLSIs guidelines.RESULT: In the present study, the highest numbers of Pseudomonas infections was found in pus followed by urine and Endotracheal secretion. Pseudomonas aeruginosa isolated from various samples were resistant to aztreonam, ciprofloxacin followed by levofloxacin, ceftazidime, cefepime, amikacin, imipenem & colistin.CONCLUSION: To prevent the spread of the resistant bacteria, it is critically important to have strict antibiotic policies wherein surveillance programmes for multidrug resistant organisms and infection control procedures need to be implemented.

Antibiogram of Pseudomonas Aeruginosa Isolates at Karaikal - Puducherry

“…This is in comparison with the studies conducted by others. 14,15 The aminoglycosides inhibit protein synthesis by binding to the30S subunit of the ribosome and the inactivation of the aminoglycosides occurs through the production of enzymes which transfer acetyl, phosphate or adenylyl groups to the amino acid hydroxyl substituents on the antibiotics. 16 When Piperacillin alone was tested, a lower rate of susceptibility (42.14%)was found in this study, whereas beta-lactam/ beta-lactamase inhibitor combination such as Piperacillin/Tazobactum showed higher susceptibility (73.58%)followed by cefoperazone/sulbactum (71.70%).…”