Chronotype is a circadian phenotype expressed in the preference of individuals to perform their activities and sleep in specific phases along the day. The objective of the study was to identify anxiety levels, quality of sleep and different chronotypes of university students and investigate their possible relationships. This is a cross-sectional study with a quantitative approach, in which 103 undergraduate students answered the Morningness-Eveningness Questionnaire (MEQ) the State and Trait Anxiety Inventory (STAI) and the Pittsburgh Sleep Quality Index (PSQI). There is a relationship between chronotype, quality of sleep and anxiety in the investigated population. Evening chronotype students showed higher anxiety status and have poor sleep quality when compared with morning chronotype students. The high occurrence of anxiety levels and poor sleep quality in evening students may be a consequence of high academic demand in a shift incompatible with the phase delay of the circadian timing system of these individuals.

Schwann cells were identified in the tumor surrounding area prior to initiate the invasion process underlying connective tissue. These cells promote cancer invasion through direct contact, while paracrine signaling and matrix remodeling are not sufficient to proceed. Considering the intertwined structure of signaling, regulatory, and metabolic processes within a cell, we employed a genome-scale biomolecular network. Accordingly, a meta-analysis of Schwann cells associated transcriptomic datasets was performed, and the core information on differentially expressed genes (DEGs) was obtained by statistical analyses. Gene set over-representation analyses was performed on core DEGs to identify significantly functional and pathway enrichment analysis between Schwann cells and, lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC). DEGs were further integrated with genomescale human biomolecular networks. miRNAs were proposed by the reconstruction of a transcriptional and post-transcriptional regulatory network. Moreover, microarraybased transcriptome profiling was performed, and the prognostic power of selected dedifferentiated Schwann cell biomolecules was predicted. We observed that pathways associated with Schwann cells dedifferentiation was overexpressed in lung cancer samples. However, genes associated with Schwann cells migration inhibition system were downregulated. Besides, miRNA targeting those pathways were also deregulated. In this study, we report valuable data for further experimental and clinical analysis, because the proposed biomolecules have significant potential as systems biomarkers for screening or for therapeutic purposes in perineural invasion of lung cancer.

California halibut Paralichthys californicus is an important commercial species with high aquaculture potential in Baja California Sur, Me´xico. To optimize the feeding process using live prey and/or inert diets, we evaluated alkaline proteases, pepsin, trypsin, chymotrypsin, leucine aminopeptidase, lipase, a-amylase, and acid and alkaline phosphatase activities on starved larvae and larvae fed live prey. Highest activities were observed for alkaline protease, trypsin, chymotrypsin, leucine aminopeptidase, and alkaline phosphatase in feeding larvae than starved larvae on day 4 after hatching. At day 5, a sizeable increase in all enzymatic activities was detected in feeding larvae. Alkaline protease, trypsin, chymotrypsin, and alkaline phosphatase decreases progressively from day 5 until day 18. At day 18, a slight pepsin activity was observed. This was considered an indicator of the start of digestive system maturation. We concluded that total enzymatic equipment for this species is complete between day 18 and 30 after hatching. Based on this evidence, early weaning from live prey to inert feed would be possible at this time.

This study assessed the effects of two artificial photoperiods (LD 24:0 and LD 12:12) and three temperature regimes (9, 11, and 18 degrees C) for 30 days on haematological parameters of trout (Oncorhynchus mykiss) kept in freshwater. Samples were taken at days 7, 14, and 30 during exposure to treatments. A higher mortality (22%) and lower oxygen concentration (<8.0 mg/l) were associated with the combination of photoperiod LD 24:0 and 18 degrees C. The LD 24:0 photoperiod (independently of temperature) increased the haematocrit and the number of erythrocytes at days 7, 14, and 30 (P < 0.01). A temperature of 18 degrees C (independently of photoperiod regimes) diminished the number of total leucocytes, lymphocytes and thrombocytes (P < 0.01). The LD 24:0 photoperiod (also independently of temperature) lowered the number of lymphocytes only after 14 days of experimentation (P < 0.01). Interaction between artificial photoperiod and temperature was only observed at day 14 for polychromatophils (P < 0.01). These results resemble the effects of stress caused by elevated temperatures and the application of continuous light photoperiods, indicating that survival risks may develop in trout farming when this combination is met.

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Abstract. the present study investigated the similarities between rodent and human urothelial carcinogenesis models using DnA content, p53 and Ki-67 immunoexpression as surrogate markers of bladder carcinogenesis. Following n-butyl-n-(4-hydroxybutyl)-nitrosamine exposure, 49 human cystectomy specimens of bladder cancer and 53 rat bladder specimens were studied. All of the tumours and adjacent mucosa present in each specimen were evaluated. High similarities were observed between the rodent urothelium carcinogenesis process and the corresponding process in humans, in regards to the histopathological features and biological alteration profile: DnA aneuploidy, p53 overexpression and high proliferative index measured by Ki-67 immunoexpression. Despite these similarities, a higher frequency of alterations was observed in earlier stages in the rat chemical-induced carcinogenesis, namely in 5c aneuploid cells, p53 overexpression and higher Ki-67 labelling index. These results confirm that this experimental animal model is a suitable and reproducible model of bladder carcinogenesis, particularly in regards to high-risk noninvasive and invasive urothelial carcinomas. these features mandate its use in the identification of new molecular targets and evaluation of tumour response to new cytotoxic drugs or drug combinations in bladder cancer therapeutic intervention. Introductionthe importance of chemical carcinogens in the development of bladder cancer has been well established and generally accepted. this carcinogenesis process occurs as a multistep process. Preneoplastic lesions, differently progressed, but with clonally related genomes, exist prior to in situ and invasive carcinoma, and may locally contribute to the independent formation of tumours (1).Animal models of cancer have been fundamental for the demonstration of this multistep nature (2). several rodent models have been established to study these different stages of urinary bladder chemical carcinogenesis, namely chemical initiation and promotion, and the stages of progression (3,4). the administration of n-butyl-n-(4-hydroxybutyl)-nitrosamine (BBn) to mice (5-7) or rats (7-11) is one of the most widely used chemical carcinogens.the resultant induced tumours in these experimental models have been histologically well studied. However, little is known regarding the biological features and genetic similarities between rodent and human bladder cancer models.this study aimed to identify biological similarities between human bladder carcinogenesis and rat chemical-induced bladder carcinogenesis by assessing DnA content alterations and p53 and Ki-67 immunoexpression, in order to use this preclinical animal model in future therapeutic experimental studies. Materials and methodsIn this study, we compared biological data collected from our previous studies, in which human (12) and rat (10) bladder samples with premalignant and malignant lesions and adjacent mucosa were studied with regards to histopathology, DnA content, p53 immunoexpression and the Ki-67 labelling index...

Circadian hygiene, a concept not to be confused with the notion of public or social hygiene, should be discussed among experts and society. Light–dark cycles and other possible synchronizers of the human circadian timing system affect ways of life, including sleeping, eating, working and physical activity. Some of these behaviors have also been investigated individually as synchronizers (e.g., eating times). Therefore, the knowledge held today about circadian rhythms, and their implications for health, allows future perspectives in this field to be mapped. The present article summarizes the latest knowledge on factors influencing circadian rhythms to discuss a perspective for the future of health promotion based on circadian hygiene. However, it is important to highlight that circadian hygiene is the product of an imbrication of individual and societal involvement. First, it is important to adopt practices and devise public health policies in line with circadian hygiene. Second, individual healthy habits require internal rhythms to be examined. Last, the research agenda on circadian hygiene can be developed on a public as well as individual level, raising the question as to how much society is willing to embrace this change.

The effects of artificial photoperiod regimes on reproductive patterns have been studied in several species, as have haematological parameters. However, information on how artificial photoperiods may affect blood components is scarce, especially under field conditions. We have assessed the effects of constant light [long day (LD) photoperiod: 24 h (light):0 h (dark)] on haematological parameters of cultured rainbow trout in Chile (Southern Hemisphere). In the initial stage (March up to June), two groups of fish were maintained under similar conditions and under the ambient (natural) photoperiod (NP). One group was then placed under the LD photoperiod regime for 2 months (June/July), following which it was returned to the NP (August-January); the control group remained under the NP throughout the experiment (March-January). All fish were assessed for haematological parameters and growth performance at various times during the experiment. During the initial stage, no differences were found between groups. However, at the end of the LD 24:0 period, the LD fish had increased haematocrit values and erythrocyte numbers and diminished mean corpuscular haemoglobin. After the LD fish had been returned to the NP, they developed secondary sexual characteristics and had a 40-44% decrease in the number of leukocytes.