BackgroundThe present study was designed to evaluate the potential of isoquercetin-based cream formulation on scald burn wound injury in rats.MethodsFour isoquercetin-based cream formulations viz. 0.01, 0.02, 0.04, and 0.06 % w/w were prepared. Cream base and standard anti-burn cream containing silver sulfadiazine were also used for comparison. Scald burn was given to rats by pouring water at 90 °C on a shaved dorsal area of 20 mm2. Deep second-degree burn injury was produced which was evaluated for the next 21 days for the percentage of wound contraction and period of epithelialization. On day 21, the rats were sacrificed and histopathological slides were prepared using hematoxylin-eosin staining. Burned tissue was also screened for levels of oxidative stress using thiobarbituric acid reactive species (TBARS) and reduced glutathione (GSH) estimation.ResultsThere was a significant increase in the percentage of wound contraction and a significant decrease in the period of epithelialization in isoquercetin-based cream-treated groups as compared with the control group. However, most significant results were obtained with isoquercetin 0.06 % w/w cream. Histologically, isoquercetin 0.06 % w/w cream treatment resulted in almost complete re-epithelialization and re-structuring of the wound tissue. There was a significant rise in TBARS and a decrease in GSH levels in the burn injury group which was reversed to a major extent by the application of isoquercetin-based cream.ConclusionsThe results indicate the wound healing potential of isoquercetin-based cream. Tissue biochemical studies indicate towards a possible role of free radical scavenging in the observed effects of isoquercetin in wound healing.
Plants synthesize compounds with biological activity, namely antioxidant, as secondary products, which are mainly phenolic compounds serving in plant defense mechanisms to counteract reactive oxygen species (ROS) in order to avoid oxidative damage [1]. Many epidemiological studies have shown that the consumption of phenolics-rich foods is associated with the prevention of chronic diseases [2]. In addition to their antioxidant properties, these compounds have been reported to be potential candidates in lowering cardiovascular diseases [3] , anticarcinogenic [4,5] , antiallergenic, antiarthrogenic, antiinflammatory, antimicrobial and antithrombotic effects [6]. Plant phenolics, in particular phenolic acids, tannins and flavonoids are known to be antioxidants and occur in vegetables, fruits, nuts, seeds, roots and barks [7]. Fertility regulation with plant or plant preparation has been revealed in indigenous system of medicines of many countries. It is difficult to establish quality control parameters of plant-based drug due to complex nature and variability of chemical constituents [8]. So, modern analytical techniques should be implicated to overcome this problem. The aim of the present study was to evaluate few antifertility herbs qualitatively and quantitatively for estimation of phytoconstituents. Ficus racemosa commonly known as Gular, Udambara belongs to the family Moraceae grows in evergreen forests, moist localities and bank of streams, deciduous forests, to the elevation of 1800 m above sea level. Well drained medium to heavy soil for successful cultivation. Propagation is done by stem and root suckers. It grows up to 18 m high [9] .
The present protocol was designed to compare the effects of Morus alba (MA) aqueous extract based cream on healing of burn wounds in rats. Fifty adult, male Wistar-albino rats were divided into five groups. The first group served as a sham control group. The animals in this group underwent shaving on the back without any burn injury. Burn was generated on the backs of all the rats in second, third, fourth and fifth group. The animals of second group served as burn injury control group and did not receive any treatment. The burned areas of the rats in the third, fourth and fifth groups were covered daily with a cream base, SSD cream and MA creasm (20% MA aqueous extract based cream) respectively. The percentage of wound contraction in a period of 11 days was observed, followed by observation in the period of epithelialization of the wound till the 21st day after injury. On the 21st day, the rats were sacrificed and the burn wound skin tissue samples were collected for histopathological examinations. Morphological and histo-pathological evaluations showed burn healing to be better in the MA and SSD groups with respect to the burn injury control group. Furthermore, it is interesting to note that the MA cream treated group had a significantly better rate of burn wound healing than the SSD group. In conclusion, application of MA cream (20% MA aqueous extract based cream) proved to be highly effective in healing burn related skin wounds in the rat model.
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