Development and validation of RP-HPLC method for the determination of stigmasterol in the botanical extract of <i>Ficus deltoidea</i>

Mukhtar, Hayat M.; Singh, Amandeep; Soni, Varinder; Singh, Atinderpal; Kaur, Hardeep

doi:10.1080/14786419.2018.1503661

Cited by 7 publications

(1 citation statement)

References 14 publications

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“…The qualitative and quantitative analysis of concentrated plant extract was carried out by following the reported liquid chromatographic methods for ursolic acid [54], stigmasterol [55] and sitosterol [56]. All the standards were adjusted equivalent to 100.0 % before use.…”

Section: Qualitative and Quantitative Analysis Of Abce By Hplcmentioning

confidence: 99%

Carbamazepine Shows Plasma and Tissue Pharmacokinetic Interactions with Ajuga bracteosa Extract in Rats

Ahmạd

Jahangir

Ishtiaq

et al. 2021

Planta Medica International Open

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Carbamazepine (CBZ) is the first-line anticonvulsant drug with a narrow therapeutic index (NTI) and is a substrate for CYP3A4 and MRP-2. Ajuga bracteosa (AB), family Lamiaceae is widely used to treat epilepsy, gastric diseases, and protects against liver damage in folk. It contains bioactive metabolites, which are powerful inhibitors of CYP3A4, CYP3A5, CYP19, CYP2C19 enzymes and P-gp transporter. Concomitant use of NTI drugs with herbs, like AB increase the chances of herb-drug interactions (HDIs). This study was aimed to analyze the Ajuga bracteosa crude extract (ABCE) and to investigate its effect on the pharmacokinetics of CBZ in rats. In the pre-treatment study, rats received ABCE (1000 mg/kg) orally for 14 days, followed by a single dose of CBZ (80 mg/kg) on the 15th day. In the co-administration study, single doses of ABCE and CBZ were administered concomitantly in one session. All the doses were administered in 0.5% carboxymethylcellulose (CMC) as a vehicle. HPLC analysis showed that extract contained 1.3 mg/g ursolic acid, 2.1 mg/g sitosterol and 2.9 mg/g stigmasterol. Non-compartmental pharmacokinetic analysis showed an increase in Cmax, AUC0-∞, MRT, and t1/2 with a decrease in tmax, Vd and Cl of CBZ in both, pre-treated and co-administered groups vs controls. An increase in CBZ concentration in liver tissue of both pre-treated as well as co-administered animals was observed as compared to control. The above results suggested possible HDIs between AB and CBZ thus, may warrant CBZ dose adjustment in epileptic patients with simultaneous administration of AB or its products.

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Section: Qualitative and Quantitative Analysis Of Abce By Hplcmentioning

confidence: 99%

Carbamazepine Shows Plasma and Tissue Pharmacokinetic Interactions with Ajuga bracteosa Extract in Rats

Ahmạd

Jahangir

Ishtiaq

et al. 2021

Planta Medica International Open

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High‐Performance Liquid Chromatography–Based Standardization of Stigmasterol in Moringa oleifera: Method Development and Validation Through Design of Experiment Approach

Kunchanur,

Mannur,

Koli

2024

Separation Science Plus

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The primary objective of this investigation was to develop and validate a robust reversed‐phase high‐performance liquid chromatography (RP‐HPLC) method for quantifying stigmasterol in both Moringa oleifera powder and tablets. The analysis was conducted using a Box–Behnken design. Stigmasterol separation was achieved with a Phenomenex Luna C‐18 column (4.6 × 150 mm2, 5 µm) and a mobile phase consisting of methanol and 0.1% formic acid in an 80:20% v/v ratio. The separation was performed at a flow rate of 1 mL/min, and stigmasterol was detected at a wavelength of 210 nm using a photodiode array detector. Method validation followed the guidelines set forth by the International Council for Harmonisation (ICH) Q2 (R1). This rigorous validation process evaluated critical parameters, including linearity, accuracy, system suitability, precision, and robustness. The results for each parameter were well within the acceptable range, confirming the reliability and efficacy of the established RP‐HPLC method. The findings of this study highlight the potential of the RP‐HPLC method as a dependable tool for the routine analysis of stigmasterol in herbal formulations.

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Isolation, identification, and quantification of stigmasterol in Hygrophila schulli plant by a validated high-performance thin-layer chromatography‒densitometric method

Takale,

Kothawale,

Ghule

et al. 2023

JPC-J Planar Chromat

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Development and validation of RP-HPLC method for the determination of stigmasterol in the botanical extract of Ficus deltoidea

Cited by 7 publications

References 14 publications

Carbamazepine Shows Plasma and Tissue Pharmacokinetic Interactions with Ajuga bracteosa Extract in Rats

Carbamazepine Shows Plasma and Tissue Pharmacokinetic Interactions with Ajuga bracteosa Extract in Rats

High‐Performance Liquid Chromatography–Based Standardization of Stigmasterol in Moringa oleifera: Method Development and Validation Through Design of Experiment Approach

Isolation, identification, and quantification of stigmasterol in Hygrophila schulli plant by a validated high-performance thin-layer chromatography‒densitometric method

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