High‐Performance Liquid Chromatography–Based Standardization of Stigmasterol in Moringa oleifera: Method Development and Validation Through Design of Experiment Approach
Mahantesh Kunchanur,
Vinodhkumar S. Mannur,
Rahul Koli
Abstract:The primary objective of this investigation was to develop and validate a robust reversed‐phase high‐performance liquid chromatography (RP‐HPLC) method for quantifying stigmasterol in both Moringa oleifera powder and tablets. The analysis was conducted using a Box–Behnken design. Stigmasterol separation was achieved with a Phenomenex Luna C‐18 column (4.6 × 150 mm2, 5 µm) and a mobile phase consisting of methanol and 0.1% formic acid in an 80:20% v/v ratio. The separation was performed at a flow rate of 1 mL/m… Show more
Set email alert for when this publication receives citations?
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.