Evaluation of the wound healing potential of isoquercetin-based cream on scald burn injury in rats

Abstract

BackgroundThe present study was designed to evaluate the potential of isoquercetin-based cream formulation on scald burn wound injury in rats.MethodsFour isoquercetin-based cream formulations viz. 0.01, 0.02, 0.04, and 0.06 % w/w were prepared. Cream base and standard anti-burn cream containing silver sulfadiazine were also used for comparison. Scald burn was given to rats by pouring water at 90 °C on a shaved dorsal area of 20 mm2. Deep second-degree burn injury was produced which was evaluated for the next 21 days for the percentage of wound contraction and period of epithelialization. On day 21, the rats were sacrificed and histopathological slides were prepared using hematoxylin-eosin staining. Burned tissue was also screened for levels of oxidative stress using thiobarbituric acid reactive species (TBARS) and reduced glutathione (GSH) estimation.ResultsThere was a significant increase in the percentage of wound contraction and a significant decrease in the period of epithelialization in isoquercetin-based cream-treated groups as compared with the control group. However, most significant results were obtained with isoquercetin 0.06 % w/w cream. Histologically, isoquercetin 0.06 % w/w cream treatment resulted in almost complete re-epithelialization and re-structuring of the wound tissue. There was a significant rise in TBARS and a decrease in GSH levels in the burn injury group which was reversed to a major extent by the application of isoquercetin-based cream.ConclusionsThe results indicate the wound healing potential of isoquercetin-based cream. Tissue biochemical studies indicate towards a possible role of free radical scavenging in the observed effects of isoquercetin in wound healing.

Liposomes with Silk Fibroin Hydrogel Core to Stabilize bFGF and Promote the Wound Healing of Mice with Deep Second‐Degree Scald

“…Scalds have some slightly distinct pathophysiological characters among all types of burns, such as a more wound exudate and necrotic debris on the surface . Scald injury tends to be the most common devastating trauma that results in either disability or death of burn patient, especially childhood . The injured depth is a significant determinant of mortality and the primary determinant of the patient's long‐term appearance and functional outcome.…”

Topical treatment with a transient receptor potential ankyrin 1 (TRPA1) antagonist reduced nociception and inflammation in a thermal lesion model in rats

“…Thus, many studies have demonstrated that the hydrogen peroxide production by tissue-resident cells (i.e., synoviocytes), macrophages and neutrophils, could favor the sensitization of nociceptors via TRPA1 activation (Moilanen et al, 2015;Trevisan et al, 2016Trevisan et al, , 2014. Previous studies reported that thermal burn might increase the reactive species production, which could contribute to the tissue damage (Bhatia et al, 2016;Hoşnuter et al, 2004;Parihar et al, 2008;Silveira et al, 2016;Tanaka et al, 1999). In this sense, antioxidants are designed to reduce production and to capture reactive species that promote tissue lesion (Wasiak et al, 2013).…”

Clinical And Experimental Applications Of The Green Nanosynthesis For Control Over The Oxidative Stress Due To Heat Burn Injury

“…Despite the major advances in the treatment of burn wounds, the systemic inflammatory response syndrome (SIRS), the sepsis and multiple organ damage are the main causes of disability and death in burns [Parihar A. et al, 2008]. In this respect, thermal burn wounds are a typical example [Abdulhalli A. et al, 2014, Bhatia N. et al, 2016.…”

“…Microcirculation is of most importance for the degree of burns. Thermal burns cause immediate and prolonged inflammatory response that generates toxins and free radicals [Parihar A. et al, 2008, Bhatia N. et al, 2014, Bhatia N. et al, 2016, Cacir B. and Yegen BC. 2004.…”