In this paper we represent a study on the potential use of protein A-tagged gold nanoparticles applied for signal amplification of electrochemical immunosensors. Gold nanoparticles (GNPs) were synthesized by the chemical reduction of tetrachloroauric (III) acid trihydrate using sodium ascorbate, and then tagged with protein A (PrA) via ultracentrifugation. UV-Vis spectroscopy and transmission electron microscopy were used to verify the characteristics of formed GNPs/PrA complex. The analyzed results indicate that GNPs were found spherically, homogeneously, and with an average diameter of about 10 nm. Immunoelectron microscopy was then used to investigate the bioactivity of the GNPs/PrA complex in solution by the effective binding of GNPs to viral particles. Scanning electron and fluorescence microscopies were also used to investigate the distribution and the bioactivity of the GNPs/PrA complex on the surface of the interdigitated sensor. Consequently, this study provided some assumptions of the potential application of protein A-tagged gold nanoparticles for signal amplification of electrochemical immunosensors in virus detection from clinical samples.
Fragment-Based Discovery of Novel and Selective mPGES-1 Inhibitors. Part 1. Identification of Sulfonamido-1,2,3-triazole-4,5-dicarboxylic Acid. -Sulfonamido-1,2,3-triazole-4,5-dicarboxylic acid derivatives (III) and (V) are synthesized using an azide-alkyne click reaction procedure. Compound (IIId) is shown to have the most inhibitory activity against mPGES-1. -(LEE, K.; PHAM, V. C.; CHOI, M. J.; KIM, K. J.; LEE, K.-T.; HAN, S.-G.; YU, Y. G.; LEE*, J. Y.; Bioorg. Med. Chem. Lett. 23 (2013) 1, 75-80, http://dx.
A New Synthetic Procedure to 2,8-Diaminoindeno[1,2-b]fluorene as a Blue Light Emitting Material. -Two synthetic routes to the title compound (V) are described. The Grignard reaction, cf. (I)→(III), and the cyclization reaction, cf. (III)→(IV), are the improvements, which overcome the problems of harsh reaction conditions and low yields. -(PHAM, V. C.; HUH, G.; KIM, J.; CHOO, D.
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