Towards the use of protein A-tagged gold nanoparticles for signal amplification of electrochemical immunosensors in virus detection

Abstract

In this paper we represent a study on the potential use of protein A-tagged gold nanoparticles applied for signal amplification of electrochemical immunosensors. Gold nanoparticles (GNPs) were synthesized by the chemical reduction of tetrachloroauric (III) acid trihydrate using sodium ascorbate, and then tagged with protein A (PrA) via ultracentrifugation. UV-Vis spectroscopy and transmission electron microscopy were used to verify the characteristics of formed GNPs/PrA complex. The analyzed results indicate that GNPs were found spherically, homogeneously, and with an average diameter of about 10 nm. Immunoelectron microscopy was then used to investigate the bioactivity of the GNPs/PrA complex in solution by the effective binding of GNPs to viral particles. Scanning electron and fluorescence microscopies were also used to investigate the distribution and the bioactivity of the GNPs/PrA complex on the surface of the interdigitated sensor. Consequently, this study provided some assumptions of the potential application of protein A-tagged gold nanoparticles for signal amplification of electrochemical immunosensors in virus detection from clinical samples.

Powerful colloidal silver nanoparticles for the prevention of gastrointestinal bacterial infections

“…Recently, several attempts have been made to control infectious pathogens and contaminations, such as development of biosensing methods for rapid detection of infectious viruses. Electrochemical DNA biosensors and immunosensors were successfully used for detection of a variety of viral pathogens such as herpes virus, Japanese encephalitis virus, or influenza A-type virus [21][22][23][24]. In addition to virus contamination detection, bacterial/viral inactivation is an option to reduce infection risk and prevention of outbreak.…”

Advances in Research on Biomedical Nanomaterials in Vietnam

“…Only one year 2012 eleven articles [1][2][3][4][5][6][7][8][9][10][11] have been published. In reference Ha P T et al [1] presented the results of their research on paclitaxel-loaded poly(lactide)-d-α-tocopheryl polyethylene glycol 1000 succinate (PLA-TPGS) nanoparticles prepared by a modified solvent extraction/evaporation technique.…”

“…The authors experimentally demonstrated the ability of paclitaxel-loaded PLA-TPGS nanoparticles to induce apoptosis in human hepatocellular carcinoma cell lines (Hep-G2), indication the potential application of these nanoparticles as a cancer chemotherapeutic agent. In reference Dang M C et al [2] reported on the fabrication of a nanoparticle formulation of ketoprofen (Keto)-encapsulated cucurbit [6] (CB [6]) uril nanoparticles, the evaluation of its in vitro dissolution and the investigation of its in vivo pharmaceutical property. The CB [6]-Keto nanoparticles were prepared by emulsion solvent evaporation method.…”

“…In reference Dang M C et al [2] reported on the fabrication of a nanoparticle formulation of ketoprofen (Keto)-encapsulated cucurbit [6] (CB [6]) uril nanoparticles, the evaluation of its in vitro dissolution and the investigation of its in vivo pharmaceutical property. The CB [6]-Keto nanoparticles were prepared by emulsion solvent evaporation method. Morphology and size of the successfully prepared nanoparticles were then confirmed using a transmission electron microscope and dynamic light scattering.…”

“…It was shown that they are spherical with hydrodynamic diameter of 200-300 nm. The in vitro dissolution studies of CB [6]-Keto nanoparticles were conducted at pH 1.2 and 7.4. The results indicated that there is a significant increase in Keto concentration at pH 7.4 compared to pH 1.2.…”