Alloplasmic lines are formed when the cytoplasm of one species is replaced by the cytoplasm of another as a result of repeated recurrent crosses of wide hybrids with the paternal genotype. Since the cytoplasm replacement results in new intergenomic interactions between a nucleus and cytoplasm leading to variability of plant characteristics, alloplasmic lines with restored fertility can be an additional source of biodiversity of cultivated plants. Earlier, recombinant alloplasmic lines (H. vulgare)-T. aestivumdesignated as L-17(1)–L-17(37) were formed from a plant with partially restored fertility of the BC3 generation of barley-wheat hybridH. vulgare(cv. Nepolegayushchii) ×T. aestivum(cv. Saratovskaya 29). This male-sterile hybrid was consistently backcrossed with wheat varieties Mironovskaya 808 (twice) and Saratovskaya 29, and Mironovskaya 808 had a positive impact on the restoration of fertility. This paper presents the results of investigation into a group of recombinant alloplasmic lines (L-17F4), as well as into doubled haploids (DH) lines – alloplasmic DH-17-lines obtained from anther culture of alloplasmic lines (L-17F2). The most productive of these lines were used as initial breeding genotypes. Hybrid form Lutescens 311/00-22 developed from the crossing of the alloplasmic DH(1)-17 line (as maternal genotype) with euplasmic line Com37 (CIMMYT), the source of the 1RS.1BL wheat-rye translocation, proved to be successful for breeding. The presence of the 1RS.1BL translocation in the genome of the Lutescens 311/00-22 form and the L-311(1)–L-311(6) alloplasmic lines isolated from it did not lead to a decrease of fertility or sterility in the plants. This indicates that the chromosome of the 1BS wheat does not carry the gene(s) that determine the restoration of fertility in the studied (H. vulgare)-T. aestivumalloplasmic lines. Alloplasmic lines L-311(1)–L-311(6) showed their advantage in comparison with the standard varieties for resistance to leaf and stem rust, yield, and grain quality. The breeding tests performed at Omsk Agricultural Scientific Center, Agrocomplex “Kurgansemena”, Federal State Unitary Enterprise “Ishimskoe” (Tyumen Region), using alloplasmic lines L-311(5), L-311(4) and L-311(6) resulted in varieties of spring common wheat Sigma, Uralosibirskaya 2 and Ishimskaya 11, respectively.
The widespread production of grain crops and the rejection of the classical technology of tillage led to a noticeable increase in the prevalence and harmfulness of phytopathogens. First of all, this applies to facultative parasites with a wide specialization - Bipolaris, Fusarium, as well as secondary fungi from the genus Alternaria, Cladosporium, Penicillium, etc. At the same time, there is a change of dominance among these species. The predominant niche is occupied by fungi of the genus Fusarium, instead of species of the genus Bipolaris. The infestation of not only the seed material with this pathogen, but also the root system requires the selection of approaches to reduce this pathogen development. The use of modern fungicides with chemical or biological substances in their composition can protect plants from the pathogen and affect yield.
Widespread production of grain crops and rejection of classical tillage technology have led to a noticeable increase of harmfulness of phytopathogens. First of all, this appertains to facultative parasites with a wide specialization - Bipolaris spp., Fusarium spp., as well as to secondary fungi from Alternaria spp., Cladosporium spp., Penicillium spp. genera and others. There is a simultaneous change of dominance in microbial community of seeds obtained in the Trans-Urals. Fungi of Fusarium spp. genus occupied the leading place among the most harmful pathogens, placing Bipolaris sorokiniana Sacc. Shoem. to the background. F. oxysporum (Schleht) Snyd, F. graminearum Schwabe, F. sporotrichoides Sherb are most spread on spring wheat, both in the tillering phase and at milk-wax stage of grain ripeness. The abundance and diversity of species was significantly higher in the tillering phase than when counted at the end of the vegetation season. To reduce the spread and development of any phytopathogen, it is necessary to apply a set of measures. One of the most effective measures is pre-sowing disinfection of seeds. However, the proposed preparations are not all equally effective in suppressing the development of the fungus of Fusarium spp. genus. During the experiment, it was found that seed treatment fungicides from the class of benzimidazoles - Zim (carbendazim), mixtures of active substances from the classes of triazoles with imidazole - HYIP (triticonazole + prochloraz), and also two triazoles - Oplot (difenoconazole + tebuconazole) were distinguished by high activity from 5.2 to 7.5 units. Application of a microbiological preparation (Bacillus subtilis), as well as its mixtures with a reduced application rate of a chemical disinfectant, showed the absence or low inhibition of fungus mycelium development.
The results of the study of the effectiveness of plant protection products against powdery mildew (Blumeria graminis) and tan spot (Pyrenophora tritici-repentis) for spring wheat are presented. It has been noted that the probability of mass destruction of crops by phytopathogens is determined by the stock of the infectious beginning of pathogens, the susceptibility of the host plant and the favorable weather conditions for their development and spread. The experiment was carried out using the methods generally accepted in the Russian Federation. During the observation period, the degree of plant damage of more than 20% in the heading phase was noted in 46% of the years of observation (in 2009, 2013, 2014, 2016, 2017). Epiphytotic development of powdery mildew together with brown leaf rust (Puccinia triticina Eriks.) was registered in 2013–2014 and 2016–2017. For 2009–2019 the degree of plant damage of more than 20% in the heading phase was noted in 46% of the years of observation (in 2009, 2013, 2014, 2016, 2017). Effective protection of wheat against powdery mildew (75–87%) was provided by fungicides based on 2–3 active ingredients. The biological effectiveness of the biofungicide (Bacillus subtilis) was manifested only in the years with moderate wheat damage and did not exceed the average level of 58%. It is more expedient to carry out operational control of yellow spotting of wheat leaves with preparations based on such active substances as "azoxystrobin + epoxiconazole", "tebuconazole + propiconazole". The level of statistically significant yield preserved due to fungicidal protection during the years of depression was 5–6%, with a moderate development of leaf diseases due to chemical fungicides - 18%, and the biological product provided an increase in productivity of 9%. During the years of epiphytoties, the chemical protection of crops retained an average of 24% of the wheat yield, polycomponent preparations had the best performance and stability of action, and the biological protection of plants under these conditions was ineffective.
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