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Microbodies were observed in the hyphal tips of all 14 fungi investigated. Their morphology varied among the fungi and their numbers were influenced by the growth medium. Microbodies were closely associated with mitochondria in one fungus and with the endoplasmic reticulum in several fungi. Catalase was not detected in microbodies with the diaminobenzidine cytochemical procedure even though catalase activity was present in extracts of these fungi. The activities of the glyoxylate-cycle enzymes isocitrate lyase and malate synthase were affected by the growth medium and were particulate in the two fungi studied by differential centrifugation. Microbodies are abundant, and they are ubiquitous among the fungi and in some cases they may contain glyoxylate-cycle enzymes.

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Refractive hexagonal inclusions in hyphae of wild-type Neurospora crassa were compared with those in two mutants deficient in or lacking ergosterol. The mutants contained more inclusions than the wild type and there were no discernible morphological differences among inclusions of the three strains. It is concluded that these inclusions do not consist of ergosterol as previously reported.

Sclerotium rolfsii secretes oxalic acid when grown on a occurred at 1.196 g/cm 3. The two key enzymes of oxalic acid medium containing 1% sodium polypectate. Mitochondria biosynthesis, glyoxylate dehydrogenase and isocitrate lyase, and microbodies were partially purified from hyphae grown had distributions similar to that of catalase, and hence appear under these conditions by centrifugation of mycelial to be localized in microbodies. No malate synthase activity homogenates on a sucrose density gradient in a zonal rotor. was detectable. This is the first report of the involvement of Peak activity of malate dehydrogenase, a mitochondrial microbodies in a process essential to the pathogenic marker, occurred at a buoyant density of 1.162 g/cm 3 , capabilities of a fungus. whereas the peak activity of catalase, a microbody marker,