Microbodies and glyoxylate-cycle enzyme activities in filamentous fungi

Maxwell, D. P.; Maxwell, M. D.; Hänssler, G.; Armentrout, V. N.; Murray, G. M.; Hoch, H. C.

doi:10.1007/bf00384753

Cited by 56 publications

(26 citation statements)

References 34 publications

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“…7) Thisˆnding sharply contrasts with the previously reported ones that either little or no activity of ICL and MS was detected from microorganisms grown in glucose-containing media, [8][9][10][11] because of catabolite repression by glucose. 3,12) Then, we have also demonstrated that MS, which coordinates with malate dehydrogenase generating NADH by oxidation of malate to oxaloacetate, plays a pivotal role in producing oxalate from glucose in the brown-rot fungus Fomitopsis palustris;…”

contrasting

confidence: 74%

Purification and Characterization of Malate Synthase from the Glucose-grown Wood-rotting BasidiomyceteFomitopsis palustris

Munir

Hattori

Shimada

2002

Bioscience, Biotechnology, and Biochemistry

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Malate synthase (EC 4.1.3.2), the key enzyme of the glyoxylate cycle, was puriˆed to a homogeneous protein from the wood-rotting basidiomycete Fomitopsis palustris grown on glucose. The puriˆed enzyme, with a molecular mass of 520 kDa, was found to consist of eight 65-kDa subunits, and to have K m of 45 and 2.2 mM for glyoxylate and acetyl-CoA, respectively. The enzyme activity was competitively inhibited by oxalate (K i , 8.5 mM) and glycolate (K i , 17 mM), and uncompetitively by coenzyme A (K i , 100 mM). The potent inhibition of the activity by p-chloromercuribenzoate suggests that the enzyme has a sulfhydryl group at the active center. However, the enzyme was inhibited moderately by adenine nucleotides and weakly by some of the metabolic intermediates of glycolysis and tricarboxylic acid cycle. The enzyme was completely inactive in the absence of metal ions and was maximally activated by Mg 2+ (K m , 0.4 mM), which also served to signiˆcantly prevent enzyme inactivation during storage.

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contrasting

confidence: 74%

Purification and Characterization of Malate Synthase from the Glucose-grown Wood-rotting BasidiomyceteFomitopsis palustris

Munir

Hattori

Shimada

2002

Bioscience, Biotechnology, and Biochemistry

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“…When expressed in P. infestans, CFP fluorescence localised to small spherical structures throughout the cytoplasm of mycelia and sporangia. They were somewhat heterogeneous in size, with a spatial distribution consistent with peroxisomes observed in electron micrographs of P. ultimum (Maxwell et al 1975). Major differences were not observed between hyphae and sporangia.…”

Section: Fusions With Markers For Subcellular Compartmentsmentioning

confidence: 57%

Vectors for fluorescent protein tagging in Phytophthora: tools for functional genomics and cell biology

Ah‐Fong

Judelson

2011

Fungal Biology

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“…This conclusion is further supported by the distinctive staining characteristics shared by the membranes of the two organelles. Microbodies have also been shown to originate from endoplasmic reticulum in castor bean endosperm (Gonzalez and Beevers 1976), and in filamentous fungi evidence of membrane connections has been indicated in Drechslera sorokiniana (Cole 1973) and Neurospora crassa (Maxwell et al 1975). A third possible role of the compact endoplasmic reticulum in E. pisi is the simultaneous dissociation of all the cisternae of the organelle resulting in the development of the dispersed form.…”

Section: Discussionmentioning

confidence: 95%

Ultrastructure of conidium development in Erysiphe pisi

Martin¹

1983

Can. J. Bot.

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MARTIN, M., and J. L. GAY. 1983. Ultrastructure of conidium development in Erysiphepisi. Can. J. Bot. 61: 2472-2495.The ultrastructure of all vegetative and reproductive cell types throughout the cycle of conidium development in Erysiphepisi has been examined using light and electron microscopy. The conidial apparatus comprises a conidiophore with, in succession above, a generative cell, and first-and second-stage conidial initials. At approximately 23°C in a 16 h light : 8 h dark period, the onset of elongation and division of the generative cells, onset of elongation of first-stage conidia, and maturation and abscission of second-stage conidia were individually synchronized in one diurnal cycle. Synchrony was also maintained between 24 and 30°C, and 12 h light : 12 h dark photoperiods but the generative cells elongated more slowly, achieving greater lengths than at lower temperatures. At 23°C in continuous light, development was not synchronized. The ultrastructure of each cell was characteristic of its type and developmental phase. Glycogen became progressively abundant in maturing conidia and occurred in all other cells except hyphal apices and generative cells. In addition to the organelles usually found in Ascomycetes, two special endomembrane components have been found. One is a reticular vacuolar system which is expanded as a major vacuole at one end. The whole system encloses a region (vacuole-associated body) 1-2 p,m across, rarely 8 p,m, which is continuous with the main ground plasm via pores in the network. The vacuole-associated bodies always lacked ribosomes but sometimes contained glycogen. The other component is a compact endoplasmic reticulum organized as spheres 0.5-5 p,m in diameter and composed of branching tubules which terminated in diverticulae and were sometimes interspersed with 8-nm filaments. Evidence for the functions of these organelles is discussed. The development and structure of the septa, of cytoplasmic components associated with conidial abscission, and of the walls of hyphae and conidia are discussed. All subaerial cell walls are covered with an osmiophilic cuticle with characteristic trilamellar structure and sometimes bearing granules and platelets. Evidence is presented that the cuticle is renewed following damage.

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Microbodies and glyoxylate-cycle enzyme activities in filamentous fungi

Cited by 56 publications

References 34 publications

Purification and Characterization of Malate Synthase from the Glucose-grown Wood-rotting BasidiomyceteFomitopsis palustris

Purification and Characterization of Malate Synthase from the Glucose-grown Wood-rotting BasidiomyceteFomitopsis palustris

Vectors for fluorescent protein tagging in Phytophthora: tools for functional genomics and cell biology

Ultrastructure of conidium development in Erysiphe pisi

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