Aim. Analysis of results of ecological and microbiological examination of territories nonwelfare for anthrax in territories of Barguzinsky and Kurumkansky districts of the Republic of Buryatia for justification of prophylaxis measures. Materials and methods. Space photographs were used and area and landscape signs of cattle grave sites were established. 174 samples of soil and 6 samples of bone remains of agricultural animals were obtained and studied. Results. Examination of 15 objects in 12 non-welfare for anthrax stationary points of the Republic of Buryatia was carried out in August 2014. A novel approach to detection of abandoned cattle grave sites, where DNA of Bacillus anthracis had been detected in samples, was developed and proposed. Conclusion. Ecological properties of soils 2 districts of the Republic facilitate prolonged conservation of B.anthracis in the environment. Measures of sanitary clean-up of non-welfare territories, utilization of biological waste and decision on legal status of abandoned objects are recommended.
Objective – study of biological and molecular-genetic properties of Bacillus anthraciscultures isolated in the supervised Siberian and Far Eastern territory between 1959 and 2013. Materials and methods. 39 B. anthracisstrains isolated from different specimens in 11 Siberian and Far Eastern constituent entities during epizootic and epidemic complications in terms of anthrax and monitoring of the soil foci were studied. The strains were examined using 16 culturalmorphological and 23 biochemical tests, for virulence, plasmid content, 15 VNTR- and 13 SNP-loci. Results and conclusions. Phenotypic and considerable genotypic heterogeneity of B. anthracisstrains found in the supervised territory was demonstrated. It was determined that 87,2 % of the isolates possessed typical cultural-morphological and biochemical properties. Twenty VNTR-profiles and three canonical SNP-clusters belonging to two global genetic branches A and B were revealed by means of molecular-genetic typing. The data can serve as a genetic marker in the investigation of anthrax cases, potential bioterrorist acts and evaluation of epizootic activity of stationary hazardous as regards anthrax areas in the specified administrative territory. The database «Biological properties of Bacillus anthracisstrains isolated in Siberia and Far East» was generated on the basis of systematization and analysis of the data concerning the origin, biological, molecular-genetic properties of the isolates. The Certificate of State Registration was also received. The database structure is replenishable and can be expanded if new technologies and methods are applied.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.