Limbah batik mengandung logam berbahaya diantaranya adalah Cr, Cu, Zn, dan Mn. Limbah batik yang dihasilkan dari industri tekstil umumnya merupakan senyawa anorganik non-biodegradable, yang dapat menyebabkan pencemaran terutama lingkungan perairan. Logam yang masuk dalam bentuk ion akan diikat oleh protein darah (Metalotionin), disebut ikatan M+Mt. Ikatan tersebut bersifat stabil dan tidak mudah lepas, sehingga memicu pembentukan radikal bebas. Radikal bebas akan menimbulkan reaksi peroksidasi lipid yang dapat merusak hati, sehingga terjadi peningkatan kadar enzim SGPT yang merupakan parameter gangguan pada fungsi hati. Ikan Nila (Oreochromis niloticus) dianggap sebagai bioindikator untuk studi toksikologi perairan dan monitoring lingkungan, mudah beradaptasi di segala kondisi lingkungan, dan memiliki nilai komersial yang tinggi. Penelitian ini dilakukan secara eksperimental dengan Rancangan Acak Lengkap (RAL), dengan perlakuan konsentrasi limbah batik Naftol 17% v/v, 34% v/v, 51% v/v, dan kontrol yang dipaparkan selama 48 jam. Analisis data menggunakan uji Anova dengan taraf kepercayaaan 95% dan dilanjutkan dengan uji Duncan. Hasil penelitian ini menunjukkan bahwa efek subletal limbah batik terhadap aktivitas enzim SGPT pada serum ikan Nila (O. niloticus) nilainya berbanding lurus. Hasil perhitungan rerata aktivitas enzim SGPT selama 48 jam pada perlakuan kontrol 6,17±0,98 U/L, konsentrasi 17% v/v yaitu 11,67±1,51 U/L, konsentrasi 34% v/v yaitu 17,67±1,51 U/L, dan konsentrasi 51% v/v yaitu 121.83±1,9 U/L. Analisis Anova (α=0,05) didapatkan nilai F hitung (199,552) > F tabel (4,30). Hasil uji Duncan menunjukkan bahwa perlakuan limbah batik dengan konsentrasi 51% v/v selama 48 jam, paling berdampak terhadap penurunan fungsi enzim SGPT.
The provision of excellent or appropriate MP-ASI will significantly affect the growth and development of children and children's intelligence. Giving MP-ASI that is not appropriate will impact problems in the nutritional status of children such as undernutrition status and overnutrition status to obesity. The introduction and provision of MP-ASI should be made gradually from liquid, semi-solid, and solid foods. This study aimed to determine the relationship between maternal education level and the accuracy of complementary feeding (MP ASI) with the nutritional status of children under five. This research is a quantitative analytical observation with a cross-sectional design. The research sample was 74 children aged 6-24 months, obtained using accidental sampling technique, data analysis using the Chi-Square test, and Fisher's alternative test. The results showed that the relationship between a mother's education level and the nutritional status of children under five was obtained with a p-value of 0.111 (p-value <0.05). The relationship between the accuracy of giving MP-ASI with the nutritional status of children under five obtained a p-value of 0.038 (p-values <0.05). This study concludes that there is no relationship between the mother's level of education with the accuracy of giving complementary feeding and the nutritional status of children under five. There is a relationship between the accuracy of giving MP-ASI with the nutritional status of toddlers.
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