Penyembuhan luka diabetes merupakan proses yang unik dan kompleks. Sejumlah dressing dikembangkan untuk mengetahui manfaat yang diharapkan meningkatkatkan proses penyembuhan. Penelitian in vivo maupun in vitro madu dan silver menunjukan hasil yang berbeda. Diperlukan penelitian lebih lanjut aktifitas sitotoksik madu dan silver terhadap sel fibroblas dalam media tinggi glukosa. Penelitian inibertujuan untuk mengetahui apakah madu dan silver bersifat sitotoksik terhadap sel fibroblast dalam media tinggi glukosa. Jenis penelitian ini adalah kuasi eksperimental dengan post test only desain. Kultur sel fibroblast di uji sitotoksik dengan meggunakan metode MTT Assay secara in vitro. Kelompok penelitian dibagi menjadi kelompok madu dengan konsentrasi 6%, 3% 1.5% dan kelompok Silver. Silver memiliki efek sitotoksik terhadap sel fibroblas dengan nilai penghambatan sebesar 100%. Madu dengan konsentrasi 6% dan 3% memiliki nilai penghambatan lebih dari 50%. Madu konsentrasi 1,5% menujukan proses penghambatan kurang dari 50% dan meningkatkan proses priliferasi sel fibroblas dalam media tinggi glukosa. Madu memiliki aktifitas sitotoksik yang lemah terhadap sel fibroblas dan dapat meningkatkan proliferasi sel, sedangkan silver memiliki aktifitas sitotoksik yang kuat terhadap sel fibroblast.
The provision of excellent or appropriate MP-ASI will significantly affect the growth and development of children and children's intelligence. Giving MP-ASI that is not appropriate will impact problems in the nutritional status of children such as undernutrition status and overnutrition status to obesity. The introduction and provision of MP-ASI should be made gradually from liquid, semi-solid, and solid foods. This study aimed to determine the relationship between maternal education level and the accuracy of complementary feeding (MP ASI) with the nutritional status of children under five. This research is a quantitative analytical observation with a cross-sectional design. The research sample was 74 children aged 6-24 months, obtained using accidental sampling technique, data analysis using the Chi-Square test, and Fisher's alternative test. The results showed that the relationship between a mother's education level and the nutritional status of children under five was obtained with a p-value of 0.111 (p-value <0.05). The relationship between the accuracy of giving MP-ASI with the nutritional status of children under five obtained a p-value of 0.038 (p-values <0.05). This study concludes that there is no relationship between the mother's level of education with the accuracy of giving complementary feeding and the nutritional status of children under five. There is a relationship between the accuracy of giving MP-ASI with the nutritional status of toddlers.
Monitor exposure from laptops or computers, cellphones and the frequency of their use, is still a suspected cause of eye disorders. The impact felt by students from monitor exposure is the appearance of eye fatigue, which results in impaired concentration and focus of learning. This study was conducted to examine the causes of eye fatigue caused by the duration, distance, time and lighting in the use of gadgets, monitor exposure. This type of the study is quantitative with an analytic observational design. The sample technique used consecutive sampling. The sample in this study was 118 Nursing students at Respati University, Yogyakarta The Bivariate test used independent T-test. The results of the study showed that the eye fatigue of students was 92.4%. The relationship test of time, distance, time, and lighting in the use of gadgets with eye fatigue obtained p value more than 0.05. The relationship between the use of eye glasses and eye fatigue was 0.008 (p value < 0.05. There are no significant different between length, distance, time and lighting in gadget use and monitor exposure and eye fatigue. There is a significant different between the used of eye glasses and eye fatigue.
Wound healing is a complex event involving both cellular and molecular activities. Fibroblasts play an important role and are keys to wound healing through cell proliferation and migration. Honey has anti-microbial, anti-oxidant, anti-inflammatory properties, which are used for various benefits such as wound healing. This study aims to explore the effect of honey on the viability and migration ability of fibroblast cells. The method used is the viability test using the MTT Assay calculated by the formula for the percentage of cell viability. Migration test using In Vitro Wound Scratch Assay. The results of the migration test images were analyzed using ImageJ. Giving honey doses of 0.5% and 0.1% increased cell viability and migration after 24 hours of intervention. Decreased cell viability after 48 hours of treatment, but there was a difference in the meaning of honey 1%, 0.5%, and 0.1% compared to control. Honey doses of 1%, 0.5%, and 0.1% increased fibroblast cell migration compared to control. The lowest honey increases the viability and migration of fibroblasts so that the possibility of wound healing. Keywords: Honey; Fibroblast Migration; Wound Scratch Assay; Wound Healing
BACKGROUND: Important stages in wound healing involve homeostasis, inflammation, proliferation, and remodeling phases. Fibroblasts are essential factors in the healing pathway through the process of cell proliferation and migration. Aloe vera contains various active compounds used for anti-inflammatory, antimicrobial, immunomodulatory, anticancer, and wound healing. AIM: This study aimed to evaluate the effect of A. vera on the viability and migration of fibroblast cells. MATERIALS AND METHODS: Fibroblasts were cultured in a monolayer with Dulbecco’s Modified Eagle Medium containing 10% fetal bovine serum, 1% pinstripe, and 0.5% fungizone. We use fresh A. vera leaves extracted with 95% ethanol. Cell viability will be evaluated using the MTT test and microscopic evaluation. Cell migration was tested using an in vitro wound scratch assay and analyzed with ImageJ software. RESULTS: A. vera stimulated cell viability compared to control (p < 0.05). Administration of A. vera does not change shape and is not toxic to fibroblasts. A. vera stimulated cell migration at doses of 250, 125, 50, and 5 μg/mL compared to control after 24 h of intervention. At 48 h incubation, migration doses of 250, 50, and 5 μg/mL were higher than control (p < 0.05). CONCLUSIONS: A. vera extract may effectively wound healing by increasing viability and migration of fibroblast cells.
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