The article discusses the treatment and prevention of feed toxicosis of pigs. A composition based on montmorillonite-containing minerals is considered as a promising enterosorbent in combination with the drug vievitin in the treatment of pig dysentery. Studies have shown that the complex use of vievitin with a sorbent by giving individually in 100 ml of drinking water at doses of 5.6 (second group) and 11.1 (third group) mg/kg body weight for vievitin and 400 mg/kg body weight bodies for the sorbent provided a high therapeutic effect in the treatment of piglets suffering from dysentery. Recovery, respectively, was 90.8 and 93.8%.
Background: Today, the urgent tasks of feeding cattle is to increase milk yield, increase the fat content in milk, and improve the quality of other animal products. However, the recommended amount of fat is not enough during calving and during lactation of cows. This leads to
the fact that the fat content of milk is reduced by 30%. The aim of the study : To study the behavior of encapsulated fats in model media corresponding to the gastrointestinal tract of cattle. Materials and methods: The study was conducted by direct gravimetric analysis using a Programix
TX 50 muffle furnace. As model media for the gastrointestinal tract of cattle, standard pH titers were used, adjusted to the required pH value with 0.1 M solutions of hydrochloric acid/sodium hydroxide. Results: After analyzing the data obtained, it was found that in a slightly alkaline
medium (pH range 7.4–7.8), the time for complete release of phospholipids is 12 hours. During experiment No. 2, the behavior of “protected fats” was revealed in model environments corresponding to physiological parameters of the gastrointestinal tract of cattle. The equivalent
amount of phosphorus to phospholipids was 11.90%, which corresponds to a proportion of the liberated phospholipids of higher fatty acids equal to 97.30%. Conclusion: “Protected fats” reach the model environment of the small intestines of ruminants in an unchanged form, where
capsule disintegration is observed, followed by the release of the phospholipid mixture. Full release of the encapsulated mixture in a slightly alkaline medium (pH 7.4–7.8) is achieved within 12 hours.
In advanced biotechnology, the utilization of enzymes to achieve new or modified compounds with antibacterial, fungicidal, and anti-cancer specifications is crucial. Mushroom lactases are a hopeful biocatalyst for the synthesis and modification of different compounds. They are an accessible and inexpensive enzyme for the preparation of reaction objects and have recently received attention. Laccase purification was performed from basidiomycete Lentinus strigosus (LS) in several stages: Stage 1. On ion-exchange chromatography on TEAE Servacell 23 (400 ml), two distinctly separated laccase activity peaks were observed, eluted from the carrier at 0.21 and 0.27 M NaCl. In order to reduce the loss of enzymes, all fractions with laccase activity were collected, concentrated, and desalted using an ultrafiltration cell (Amicon, United States) with a UM-10 membrane. Stage 2. The resulting preparation with laccase activity was applied to a Q-Sepharose column (60 ml). Two well-separated peaks with laccase activity were obtained during the elution: laccase I (0.12 M NaCl) and laccase II (0.2 M NaCl). Stage 3. In the course of further purification of both enzymes, carried out on anion-exchange carrier Resource Q (6 ml), a broken gradient was used: 0 - 10%, 10 - 20%, and 20 - 100% with 1M NaCl. Stage 4. Both laccase I and laccase II, obtained after Resource Q, were desalted, concentrated to 1 ml each, and applied to a Superdex 75 gel filtration column. As a result, two laccases were obtained in a homogeneous form.
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