A temporary inhibition of RNA synthesis leads to a corresponding delay in the formation of all puffs which are stimulated by ecdysone in the salivary gland chromosomes of Chironomus tentans. Inhibition of protein synthesis does not influence the induction of those puffs which appear shortly after injection of ecdysone. Puffs which develop after a longer period are delayed in appearance. It is concluded that early reacting genes are involved in those processes leading to the sequential activation of the puffs which appear later.
In cells of salivary glands of last-instar larvae of Chironomus tentans, acid phosphatase activity is bound to (probable) lysosomes and a few other cell organelles. At the end of the pupal molt the salivary gland breaks down. While acid phosphatase in areas of nondegenerated cells is still restricted to the structures mentioned, in degenerated areas the enzyme is freely distributed in the cytoplasm.
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