Lysosomal and Free Acid Phosphatase in Salivary Glands of
 Chironomus tentans

Schin, Ki Ssu; Clever, Ulrich

doi:10.1126/science.150.3699.1053

Cited by 79 publications

(25 citation statements)

References 7 publications

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“…Autolysis and phagocytosis of degenerating cells have been studied in some insects. Lysosomal acid phosphatase activity was found in the prothoracic glands of Leucophaea maderae (Scharrer 1966, Osinchak 1965, in the salivary gland of Chironomus tentans (Schin and Clever 1968) The assumption is made from the present study that structural charges and acid phosphatase activity occurring during the stagesof development of these eye discs are related to functional changes of lysosomes.…”

Section: Discussionmentioning

confidence: 50%

FINE STRUCTURAL AND CYTOCHEMICAL STUDIES OF THE DEVELOPING BAR EYE DISCS IN DROSOPHILA MELANOGASTER

Michinomae

1974

The Japanese journal of genetics

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Section: Discussionmentioning

confidence: 50%

FINE STRUCTURAL AND CYTOCHEMICAL STUDIES OF THE DEVELOPING BAR EYE DISCS IN DROSOPHILA MELANOGASTER

Michinomae

1974

The Japanese journal of genetics

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“…[53][54][55][56][57] 20-OHE also has a strong influence in the regulation of the synthesis and activity of the lysosomal enzymes. 26,36,[58][59][60][61] The main aim of the present work was to select and identify autophagy-related genes in Drosophila melanogaster. Late larval lethal strains generated by P-element mutagenesis were screened to find mutants showing defects in the formation of autophagic structures (acidic granules identified by light and electron microscopy) in the larval fat body cells.…”

Section: Discussionmentioning

confidence: 99%

“…More than half of them have proven to possess the same or similar roles in autophagy in plants, nematodes, flies and mammals. 24 Insect metamorphosis represents one of the most extreme examples for this process, since in most larval tissues a mass of autophagic vesicles is observable before and during the destruction of the epidermis, 25 salivary gland, 26 larval midgut, 27,28 Malpighian tubes, 29 prothoracic glands 30 and fat body. 31,32 These vesicles appear in a well determined time of the postembryonal development and their appearance is under the control of the insect molting hormone.…”

Section: Introductionmentioning

confidence: 99%

SNF4Aγ, the Drosophila AMPK γ subunit is required for regulation of developmental and stress-induced autophagy

et al. 2008

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In holometabolous insects including Drosophila melanogaster a wave of autophagy triggered by 20-hydroxyecdysone is observed in the larval tissues during the third larval stage of metamorphosis. We used this model system to study the genetic regulation of autophagy. We performed a genetic screen to select P-element insertions that affect autophagy in the larval fat body. Light and electron microscopy of one of the isolated mutants (l(3)S005042) revealed the absence of autophagic vesicles in their fat body cells during the third larval stage. We show that formation of autophagic vesicles cannot be induced by 20-hydroxyecdysone in the tissues of mutant flies and represent evidence demonstrating that the failure to form autophagic vesicles is due to the insertion of a P-element into the gene coding SNF4Aγ, the Drosophila homologue of the AMPK (AMP-activated protein kinase) γ subunit. The ability to form autophagic vesicles (wild-type phenotype) can be restored by remobilization of the P-element in the mutant. Silencing of SNF4Aγ by RNAi suppresses autophagic vesicle formation in wildtype flies. We raised an antibody against SNF4Aγ and showed that this gene product is constitutively present in the wild-type larval tissues during postembryonal development. SNF4Aγ is nearly absent from the cells of homozygous mutants. SNF4Aγ translocates into the nuclei of fat body cells at the onset of the wandering stage concurrently with the beginning of the autophagic process. Our results demonstrate that SNF4Aγ has an essential role in the regulation of autophagy in Drosophila larval fat body cells.

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“…For instance, in the 1970s it was observed that in Lepidoptera the normal degeneration of intersegmental muscles-an essential step in insect metamophosis-happens through autophagy 111 in the same way the regression of the salivary glands of several insects including Drosophila is also happening through autophagy. [112][113][114] But it is only recently that autophagy's role in development is being investigated more deeply. A crucial function of autophagy in early mammalian preimplantation development has been shown: maternal proteins and RNAs in oocytes are degraded by autophagy in order to activate zygotic transcription and translation.…”

Section: Why Study Autophagy In Planarians?mentioning

confidence: 99%

Autophagy meets planarians

González‐Estévez

2009

Autophagy

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This review aims to demonstrate the importance of freshwater planarians as model organisms, particularly emphasizing those characteristics of the animal that make them a good model to study autophagy. The aim of this review is to provide a better understanding of autophagy in this model for the nonplanarian reader, and elucidate the relevance of autophagy research in this peculiar model organism. Furthermore, I will try to synthesize the evidence showing the importance of autophagy in planarian body remodeling, and I will discuss some ideas about the role of autophagy in stem cell biology. In light of these new developments, it is likely that the planarian field will make an important contribution to the study of the molecular mechanisms involved in autophagy in the future.

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Lysosomal and Free Acid Phosphatase in Salivary Glands of Chironomus tentans

Cited by 79 publications

References 7 publications

FINE STRUCTURAL AND CYTOCHEMICAL STUDIES OF THE DEVELOPING BAR EYE DISCS IN <i>DROSOPHILA MELANOGASTER</i>

FINE STRUCTURAL AND CYTOCHEMICAL STUDIES OF THE DEVELOPING BAR EYE DISCS IN <i>DROSOPHILA MELANOGASTER</i>

SNF4Aγ, the Drosophila AMPK γ subunit is required for regulation of developmental and stress-induced autophagy

Autophagy meets planarians

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