Lead induced oxidative stress contributes to increase the productivity of reactive oxygen species (ROS) and to disrupt the antioxidant balance. Gynura procumbens adventitious root (GPAR) methanol extract contains abundant phenolic and flavonoids compounds as antioxidants and can be used as traditional medicinal plants. The objective of this study is to evaluate the protective effect of GPAR against lead acetate toxicity in mice to haematological parameter, histological of hepatic cells, and activities of antioxidant enzymes. The data obtained from five groups of treatment: P1 (control), P2 (Pb acetate-100 mg/L), P3 (GPAR-100 mg/L + Pb acetate-100 mg/L), P4 (GPAR-200 mg/L + Pb acetate-100 mg/L), P5 (GPAR-300 mg/L + Pb acetate-100 mg/L). The results indicated that administration of methanol extract of GPAR can prevent the decreasing of haematological parameter, maintain the percentage of normal hepatic cells, activities of superoxide dismutase (SOD), and catalase (CAT) due to lead acetate treatment. The effective dose of GPAR extract was 300 mg/L. This study provides that methanol extract of G. procumbens adventitious root exerts protective effects against lead acetate toxicity in mice.
Exposure to cadmium (Cd) could increase of reactive oxygen species (ROS) and changes in expression of antioxidant genes. Gynura procumbens is a medicinal plant that is rich in phenolic and flavonoid compounds. The aimed of study to evaluate the hepatoprotective effect of G. procumbens adventitious root (GAR) extract against Cd toxicity, especially expression rate of hepatic antioxidant genes. Twenty-five male mice were treated as follows: P1 (control), P2 (Cd100mg/L), P3 (GAR100mg/L + Cd100mg/L), P4 (GAR200mg/L + Cd100mg/L), and P5 (GAR300mg/L + Cd100mg/L). The samples (blood and liver) were collected for analysis of malondialdehyde (MDA) levels, superoxide dismutase (SOD) and catalase (CAT) activities, and their relative gene expression were determined. The hematological assay showed Cd-treated administered with GAR extract increased the number of red blood cells (RBC), haemoglobin concentration (HGB), hematocrit (HCT), mean corpuscular volume (MCV), and mean corpuscular haemoglobin (MCH), but reduced the level of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). In addition, the GAR extract decreased the MDA production, but increased the activities of SOD and CAT. These enzymatic activities were positively correlated with their respective gene transcripts. Our study revealed that GAR extract administration showed marked hepatoprotective effects against Cd-induced oxidative stress.
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