Gynura procumbens Adventitious Root Extract Altered Expression of Antioxidant Genes and Exert Hepatoprotective Effects Against Cadmium-Induced Oxidative Stress in Mice

Abstract

Exposure to cadmium (Cd) could increase of reactive oxygen species (ROS) and changes in expression of antioxidant genes. Gynura procumbens is a medicinal plant that is rich in phenolic and flavonoid compounds. The aimed of study to evaluate the hepatoprotective effect of G. procumbens adventitious root (GAR) extract against Cd toxicity, especially expression rate of hepatic antioxidant genes. Twenty-five male mice were treated as follows: P1 (control), P2 (Cd100mg/L), P3 (GAR100mg/L + Cd100mg/L), P4 (GAR200mg/L + Cd100mg/L), and P5 (GAR300mg/L + Cd100mg/L). The samples (blood and liver) were collected for analysis of malondialdehyde (MDA) levels, superoxide dismutase (SOD) and catalase (CAT) activities, and their relative gene expression were determined. The hematological assay showed Cd-treated administered with GAR extract increased the number of red blood cells (RBC), haemoglobin concentration (HGB), hematocrit (HCT), mean corpuscular volume (MCV), and mean corpuscular haemoglobin (MCH), but reduced the level of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). In addition, the GAR extract decreased the MDA production, but increased the activities of SOD and CAT. These enzymatic activities were positively correlated with their respective gene transcripts. Our study revealed that GAR extract administration showed marked hepatoprotective effects against Cd-induced oxidative stress.

Optimization of Biomass and Secondary Metabolite Production in Gynura procumbens (Lour.) Merr. Adventitious Roots Culture by Using the Method of Subculture and Fed-batch Cultivation in a Bioreactor

“…Previous works have reported on the pharmaceutical activities of G. procumbens extract, especially their positive effect on human health (Takanashi et al 2019). Recent studies have shown that in vitro roots of G. procumbens have excellent antioxidant activity and hepatoprotective effects (Sugiharto et al 2021(Sugiharto et al , 2022. Furthermore, G. procumbens is a safe and edible plant because it does not contain toxic compounds like, pyrrolizidine alkaloids (Saiman et al 2012).…”

An in vivo and in silico evaluation of the hepatoprotective potential of Gynura procumbens: A promising agent for combating hepatotoxicity

“…For this reason, the Chinese name of this plant is "Pokok Sambung Nyawa," the meaning of which is "prolongation of life" [37,48]. Furthermore, certain findings have provided significant evidence demonstrating the promising hepatoprotective potentials of G. procumbens, which has piqued the interest of researchers to investigate further the protective effects of G. procumbens against specific types of hepatotoxicity [24,49]. Hence, the present study aims to evaluate the hepatoprotective activities of G. procumbens against CCl 4 -induced hepatotoxicity, and histopathological studies, and computationally predict the ADMET properties of its phytoconstituents.…”