Background Association of single nucleotide polymorphisms (SNP) rs7756992 A/G and rs7754840 G/C of cyclin-dependent kinase 5 regulatory subunit-associated protein 1-like 1 (CDKAL1) gene with the susceptibility of gestational diabetes mellitus (GDM) has been studied in a group of Bangladeshi women. Methods In this case–control study, 212 GDM patients and 256 control subjects were genotyped for rs7756992 and rs7754840 by PCR-RFLP and TaqMan™ allelic discrimination assay method respectively. Genotyping results were confirmed by DNA sequencing and replicated TaqMan™ assay. The odds ratios and their 95% confidence interval were calculated by logistic regression to determine the associations between genotypes and GDM. Results The genotype frequencies of rs7756992-AA/AG/GG in the GDM group and the control group were 37%/48%, 53%/45%, 10%/7% and those of rs7754840-CC/CG/GG were 51%/55%, 40.1%/39.8%, 9%/5% respectively. Under dominant and log additive models rs7756992 was revealed significantly associated with GDM after being adjusted for family history of diabetes (FHD) and gravidity. Conversely, rs7754840 was significantly associated (P = 0.047) with GDM only under the recessive model after the same adjustment. The risk allele frequency of both SNPs was higher in the GDM group but significantly (P = 0.029) increased prevalence was observed in the rs7756992 G allele. When positive FHD and risk alleles of these SNPs were synergistically present in any pregnant woman, the chance of developing GDM was augmented by many folds. The codominant model revealed 2.5 and 2.1 folds increase in odds by AG (rs7756992) and GC (rs7754840) genotypes and 3.7 and 4.5 folds by GG (rs7756992) and CC (rs7754840) genotypes respectively. A significant 2.7 folds (P = 0.038) increase in odds of GDM resulted from the interaction of rs7756992 and family history of diabetes under the dominant model. The cumulative effect of multigravidity and risk alleles of these SNPs increased the odds of GDM more than 1.5 folds in different genotypes. Conclusion This study not only revealed a significant association between rs7756992 and rs7754840 with GDM but also provided the possibility as potential markers for foretelling about GDM and type 2 diabetes mellitus in Bangladeshi women.

Background Type 2 diabetes mellitus (T2DM) is a chronic disorder of which stress is a major contributor. Under stressful condition, body synthesizes a family of molecular chaperone called Heat‐shock proteins (HSPs). Current study assessed the frequency and association of HSP70‐hom + 2,437 T/C polymorphism with T2DM risk among Bangladeshis. Methods This polymorphism was selected through bioinformatics analyses and identified by PCR‐RFLP method. Results Bioinformatics analysis identified this SNP as missense mutation which could destabilize the final HSP product. Heterozygous mutant (CT) genotype was significantly associated with T2DM incidence among the studied populations (p = .015). Further analysis revealed a strong association with female patients (p = .002), while the male group showed no association (p = .958). Moreover, the C allele was significantly associated among all diabetic patients (p = .016) and particularly in the female patient group (p = .001). However, under stressful condition, males with CT genotype were at high risk for T2DM incidence whereas, females with CT genotype showed no significant association. Conclusions HSP70‐hom + 2,437 T/C polymorphism was found to be significantly associated with T2DM incidence in the Bangladeshi population in both stress‐dependent and independent manners.

No abstract

Background This study aimed to investigate the potential of High-Intensity Interval Training (HIIT) as a non-pharmacological intervention to reduce the risk of cardiovascular disease in a specific population. Methods A quasi-experimental design was employed; involving 20 young adults aged 25-30 recruited from a fitness centre. The participants underwent an 8 weeks high-intensity exercise program consisting of 3 weekly sessions. Baseline measurements of body mass, height, BMI, cholesterol, triglycerides, LDL, and HDL levels were taken before the intervention. Post-intervention measurements were obtained at the end of the 8 weeks. Results The results demonstrated significant improvements in various parameters following the 8 weeks workout program. BMI decreased from 32.2±1.42 to 31.67±1.45, while cholesterol levels decreased from 221.37±9.3 to 201±9.64, indicating a reduction in total cholesterol. Triglyceride levels decreased from 181.5±10.98 to 170.1±11.93. LDL values decreased from 144.5±8.9 to 134.2±8.13, indicating a decrease in low-density lipoprotein. Additionally, HDL levels increased from 36.53±4.53 to 46±5.44, reflecting an increase in high-density lipoprotein levels. All these changes were statistically significant (p≤0.005). Conclusion The findings suggest that HIIT is an efficient and effective exercise for sedentary and inactive young males. The study supports using HIIT as a non-pharmacological approach to improve physical well-being, enhance fitness, and reduce the risk of cardiovascular diseases. HIIT provides a time- and cost-efficient alternative for individuals with limited exercise time who still desire optimal health and fitness outcomes. Keywords Cardiovascular Diseases, High-Intensity Interval Training, Sedentary Lifestyle, Young Adults.