Summary
The radioallergosorbent test (RAST) was performed with a purified allergen (DS 22) from cod, coupled to CNBr‐activated cellulose discs. A 100% correlation was found between a positive RAST and the presence of clinical allergy to cod in fifty‐six children as compared to a negative RAST in all sera from fifty‐six allergic children tolerating fish. Positive results were obtained with discs treated with DS 22 in a concentration as low as 0.4 ng/ml.
Rabbit antiserums were prepared against three submicrosomal fractions from liver of normal or phenobarbital-treated rats. The two membrane fractions originating from the rough- and smooth-surfaced endoplasmic reticulum were characterized by the same soluble antigens, with the exception of a highly basic coinponent present only in extracts of rough membranes. The third fraction, whose subcellular origin is unknown, was different. It contained at least two typical marker antigens not present in the other fractions. Of eight tissue antigens common for the endoplasmic reticulum, five displayed nonspecific esterase activity. Some of these esterases were also found in other organs, but none was seen in rat serum. Phenobarbital treatment of the rats led to a rise in activity and characteristic changes in the esterase patterns of all these submicrosomal liver fractions.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.