The radioallergosorbent test with a purified allergen from codfish

Abstract

Summary The radioallergosorbent test (RAST) was performed with a purified allergen (DS 22) from cod, coupled to CNBr‐activated cellulose discs. A 100% correlation was found between a positive RAST and the presence of clinical allergy to cod in fifty‐six children as compared to a negative RAST in all sera from fifty‐six allergic children tolerating fish. Positive results were obtained with discs treated with DS 22 in a concentration as low as 0.4 ng/ml.

Diagnosis of Reaginic Allergy with House Dust, Animal Dander and Pollen Allergens in Adult Patients

“…Other studies have clearly shown that purified allergens increase the precision of RAST [2,3,15] and the varying precision of RAST has highlighted the unsatisfactory quality of many allergen extracts. It is obvious that if an extract contains a high amount of nonallergenic components with an affinity for the activated sites similar to or higher than the allergen components then this will result in a low allergen representation on the test particles.…”

“…Various degrees of relationship have been noted between in vivo and in vitro determinations, to some extent depending on the types of allergens used. Thus, a 100% correlation has been found with the purified and well-character ized cod allergen [3,15] while only 59% agreement was found utilizing house dust extract as allergen [2], Three studies with several allergens on randomly selected ma terial from children have revealed agree ments between RAST and PT of 84%> [6] and 77% [8,9].…”

“…The isola tion and characterization of this immuno globulin class made it possible to develop a discriminative and sensitive in vitro assay, the radioallergosorbent test (RAST) for de tection and quantitation of the IgE antibod ies against various allergens [27]. The in vi tro results obtained with the RAST have been related to results from in vivo diagnos tic tests such as skin tests (ST) and provoca tion tests (PT) which have been performed mainly with selected patient materials or with a limited number of allergens [2,3,11,14,16,17,20,21,[23][24][25][26][27][28][29][30][31][32][33]. Various degrees of relationship have been noted between in vivo and in vitro determinations, to some extent depending on the types of allergens used.…”

A Modern Clinical Approach to Food Hypersensitivity

“…When a food has been avoided for a long period and antigenic stimulation has been lacking, the level of specific IgE in the serum by RAST may be low or undetectable; after ingestion of the food the serum level and RAST value may increase considerably fi-om a booster effect of antigen in a sensitized person (54). These may be explanations for the finding that the skin test seems to be superior to RAST for detection of reaginic sensitization to food antigens and identification of persons who will develop symptoms in food challenges (64)(65)(66)(67).…”

Isolation and Characterization <i>in vitro</i> of an Allergen from Plane-Tree <i>(Platanus acerifolia)</i> Pollen

“…Many of them possess similar physicochemical properties (e.g., ac idity) [1,4], Whole pollen extracts are characterized by a heterogeneity in their protein content, many of which are not allergenic. This is true for ragweed as well as for grass pollen [9,10,12,14].…”