We have examined the motility, morphology, and cryopreservation of epididymal spermatozoa collected by needle biopsy from cynomolgus monkeys (Macaca fascicularis). At collection, epididymal sperm (23 x 10(6) +/- 4 x 10(6) sperm/sample; 611 x 10(6) +/- 116 x 10(6) sperm/ ml; n = 18) were alive (79 +/- 2%), motile (67 +/- 2%), and exhibited intact membranes (65 +/- 2%). Sperm maintained at room temperature in handling medium exhibited decreased motility over time, but head-to-head agglutination was limited. Tris egg-yolk extender containing 6% glycerol and dimethylsulfoxide (DMSO) did not significantly affect functional morphology, whereas extender containing propanediol significantly reduced motility, survival, and membrane integrity. Cryostorage reduced all measures of functional morphology independent of cryoprotectant. Post-thaw motility was superior for glycerol and DMSO compared to propanediol. Variation in glycerol concentration (4, 6, and 8%) produced equivocal effects on sperm functional morphology post-thaw. Needle biopsy may be a useful technique for laboratory and field-based collection of spermatozoa from nonhuman primates.

The aims of this study were to investigate the freezing capability of Pasundan bull spermatozoa in Tris-egg yolk (TEY), Tris-soy (TS), and AndroMed ® as diluents. Semen were collected twice a week from four Pasundan bulls aged 3-5 years old using an artificial vagina and evaluated macro-and microscopically. Semen had ≥70% sperm motility, ≥800x10 6 /mL sperm concentration, and less than 20% sperm abnormalities were divided into three parts and each of them diluted with TEY, TS, or AndroMed ® . After an equilibration step at 5° C for four hours, diluted semen were packaged in 0.25 mL straw, frozen in liquid nitrogen for ten minutes and kept in liquid nitrogen container until examination. Motility test on fresh, diluted, equilibrated, and after-thawed semen was done using Androvision ® . The results showed that after thawing motility of sperm diluted in AndroMed ® (58.64±0.72%) was higher than in TEY (49.45±1.22%) and TS (39.34±6.33%). Sperm motility of Pasundan bulls diluted in these three diluents reduced around 33.27±2.45% during freezing process. ____________________________________________________________________________________________________________________ ABSTRAKPenelitian ini bertujuan mengetahui kemampuan bertahan spermatozoa sapi pasundan dalam proses pembekuan menggunakan pengencer tris kuning telur (TKT), tris soya (TS), dan AndroMed ® . Empat ekor pejantan sapi pasundan dengan umur sekitar 3-5 tahun dikoleksi semennya menggunakan vagina buatan dua kali dalam seminggu. Semen hasil koleksi segera dievaluasi secara makroskopis dan mikroskopis. Semen dengan motilitas ≥70%, konsentrasi ≥800x10 6 /ml, dan abnormalitas spermatozoa ≤20% dibagi tiga bagian, masing-masing diencerkan menjadi 100x10 6 /ml, menggunakan pengencer TKT, TS, atau AndroMed ® , diekuilibrasi pada suhu 5° C selama empat jam, dan dikemas dalam straw 0,25 ml. Semen yang telah dikemas dibekukan dalam uap nitrogen cair selama sepuluh menit dan disimpan dalam kontainer untuk pengujian lebih lanjut. Pengujian motilitas dilakukan menggunakan Androvision ® pada semen segar, setelah pengenceran, setelah ekuilibrasi, dan post thawing. Hasil penelitian menunjukkan motilitas spermatozoa post thawing dalam pengencer AndroMed ® (58,64±0,72%) lebih tinggi dibandingkan pengencer TS (39,34±6,33%), namun tidak berbeda dengan TKT (49,45±1,22%). Pengencer TKT tidak memperlihatkan perbedaan (P>0,05) dengan TS. Penurunan motilitas selama proses pembekuan pada sapi pasundan adalah sebesar 33,27±2,45%. ____________________________________________________________________________________________________________________ Kata kunci: andromed, freezing capability, sapi pasundan, tris kuning telur, tris soya

No abstract

No abstract

Stages of the seminiferous epithelium of the testis of the wild Javan muntjac (Muntiacus muntjak muntjak) in hard antler period were characterized based on the tubular morphology method. The number and the relative frequencies of seminiferous epithelium stages and the morphometry of germinal cell nuclei were identified microscopically. We identified eight stages of seminiferous epithelium in testicular tissue of the Javan muntjac and found that the relative frequencies of stages I to VIII were 14.87, 15.12, 17.75, 6.87, 7.37, 12.37, 13, and 12.62%, respectively. The diameter of the nuclei of germinal cells varied in each stage of seminiferous epithelium. Diplotene-stage primary spermatocytes had prominent and large nuclei ~8.97 ± 1.0 μm in stages III and IV. Pachytene primary spermatocytes appeared in most stages, except stage IV, whereas leptotene- and diplotene-stage primary spermatocytes were found in stages I and II, and III and IV, respectively. Round spermatids were observed in stages IV to VIII and in stage I but were absent in stages II and III, while elongated spermatids were observed in all stages except stage I. Our findings show that the stages of seminiferous epithelium in the Javan muntjac are similar to those found in neotropical cervids, small ruminants, and other domestic animals.

The aims of study was to compare the glycerol concentration in Tris glucose egg yolk (TGEY) diluents on the quality of deer frozen semen. Semen was collected from 5 Timor deer using electroejaculator. Immediately after collection the semen was evaluated macroscopic and microscopically. After initial evaluation, the semen was divided into three tubes and extended with Tris egg yolk with three different glycerol concentrations, which were 10% (TGEY 10); 12% (TGEY 12) and 14% (TGEY 14). The sperm motility, viability, acrosome intact and membrane intact were evaluated in raw semen, after equilibration and after thawing. The results showed that there were no differences (p>0.05) on the sperm motility, viability as well as sperm acrosome intact. Sperm membrane intact in TGEY 10 (52.50±5.89%) and TGEY 14 (51.50±4.12 %) were higher (p<0.05) than in TGEY 12 (49.00±6.58). It was concluded that 10, 12 or 14% glycerol concentration can be used for Timor deer semen cryopreservation.

Penelitian ini bertujuan untuk mempelajari morfologi kelenjar aksesori muncak jantan secara makroanatomi dan mikroanatomi. Seekor muncak jantan dewasa berumur 4-5 tahun dengan bobot badan 19 kg digunakan pada penelitian ini. Muncak terlebih dahulu di-exanguinasi untuk dikoleksi kelenjar aksesori kelaminnya. Untuk memperoleh gambaran mikroanatomi, sampel kelenjar aksesori diproses dengan teknik histologi dan diwarnai dengan pewarnaan hematoksilin-eosin (HE). Hasil pengamatan makroskopis menunjukkan bahwa kelenjar aksesori muncak jantan terdiri atas ampula, duktus deferens, kelenjar prostat, kelenjar vesikularis, dan kelenjar bulbouretralis. Karakteristik histologi kelenjar aksesori muncak adalah ditemukannya kelenjar prostat yang berbentuk pars diseminata dengan kelenjar-kelenjar sekretori tersebar di sekeliling lumen uretra pars pelvina dimana secara makroskopis kelenjar tersebut tidak dapat diamati. Tipe kelenjar sekresi pada ampula, kelenjar vesikularis, dan pars diseminata prostat adalah tubuloalveolar, sedangkan pada kelenjar bulbouretralis tipe tubular. Dapat disimpulkan bahwa morfologi kelenjar aksesori muncak jantan memperlihatkan kemiripan dengan kelenjar aksesori pada ruminansia kecil lainnya seperti kambing, domba, reeves muntjak, dan pampas deer.

Tujuan penelitian ini adalah untuk menguji efek antioksidan astaxanthin dan glutathione dalam pengencer Ringer Laktat Kuning Telur (RL-KT) pada kualitas semen cair ayam merawang, SK kedu, dan kampung. Masing-masing 5 ekor ayam merawang, SK kedu, dan kampung, berumur 1-1,5 tahun digunakan sebagai sumber semen. Penelitian ini terdiri atas 2 tahap: (I) penelitian pertama bertujuan untuk menentukan dosis terbaik antioksoidan astaxanthin (0,004 %, 0,005 %) dan glutathione (0,007%, 0,008 %) pada motilitas spermatozoa dengan menggunakan lima ekor ayam merawang. (II) penelitian kedua bertujuan menguji dosis antioksidan terbaik yang didapatkan dari penelitian pertama pada motilitas dan viabilitas spermatozoa pada 3 jenis ayam lokal. Hasil penelitian pertama menunjukkan tidak ada perbedaan pengaruh antara dosis astaxanthin dan glutathione dalam pengencer RL-KT yang disimpan selama 60 jam, namun spermatozoa pada dosis astaxanthin 0,004% (3900±3,3%) menunjukkan motilitas yang lebih baik sehingga digunakan pada penelitian kedua. Hasil penelitian kedua menunjukkan bahwa penambahan astaxanthin 0,004% dapat mempertahankan motilitas spermatozoa yang disimpan sampai 36 jam pada ayam SK kedu (40,10±1,33%) dan kampung (41,03±2,44%) dan 24 jam pada ayam merawang (46,41±4,42%). Viabilitas spermatozoa pada ketiga jenis ayam tergolong tinggi, yaitu berkisar antara 59,37±5,65% sampai 65,35±6,25%. Dari data yang diperoleh, volume dan konsentrasi spermatozoa ayam merawang lebih tinggi dibandingkan SK kedu dan kampung. Semen diketahui mampu bertahan dalam waktu 36 jam pada pengencer RL-KT dengan penambahan astaxanthin 0,004% dengan motilitas mencapai 40%. Pengencer RL-KT yang ditambahkan astaxanthin 0,004% menghasilkan rata-rata fertilitas spermatozoa sebesar 87%.