SK Kedu Semen Cryopreservation in Beltsville Poultry Semen Extender and Lactated Ringer’s-Egg Yolk Extender Using Dimethyl Sulfoxide

Telnoni, Sipora Petronela; Arifiantin, R. Iis; Yusuf, Tuty Laswardi; Darwati, Sri

doi:10.3923/ajpsaj.2017.14.19

Cited by 8 publications

(10 citation statements)

References 15 publications

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“…The type of diluent, as well as the type and inclusion level of cryoprotectant, may be modified again to obtain better results (Gerzilov, 2010;Rakha et al, 2018;Di Iorio et al, 2020). Previously reported studies on rooster semen cryopreservation showed that the inclusion levels of DMSO or glycerol can vary depending on the cryopreservation procedure and the animal breed used, suggesting that a single protocol will not always work for all breeds (Blanch et al, 2014;Khaeruddin et al, 2019;Long et al, 2010;Pelaez et al, 2011;Svoradová et al, 2018;Telnoni et al, 2017). In rooster semen cryopreservation, a higher inclusion of DMSO [i.e., 7.0% (Khaeruddin et al 2019) and 10.0% DMSO (Telnoni et al, 2017)] was used.…”

Section: Discussionmentioning

confidence: 99%

“…Previously reported studies on rooster semen cryopreservation showed that the inclusion levels of DMSO or glycerol can vary depending on the cryopreservation procedure and the animal breed used, suggesting that a single protocol will not always work for all breeds (Blanch et al, 2014;Khaeruddin et al, 2019;Long et al, 2010;Pelaez et al, 2011;Svoradová et al, 2018;Telnoni et al, 2017). In rooster semen cryopreservation, a higher inclusion of DMSO [i.e., 7.0% (Khaeruddin et al 2019) and 10.0% DMSO (Telnoni et al, 2017)] was used. An inclusion rate of 10.0% DMSO also worked well in Jinding duck semen's cryopreservation, but in a different extender (Han et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

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Superoxide Dismutase (SOD) Activity in Cryopreserved Semen of Itik Pinas-Khaki (Anas platyrhynchos L.)

Ancuelo

Landicho

Dichoso

et al. 2021

Trop. Anim. Sci. J.

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Cryopreservation induces oxidative stress on sperm due to an increase in the number of reactive oxygen species (ROS), thereby resulting in decreased sperm quality. ROS's destructive potential is normally counteracted in sperm by their innate antioxidant system consisting of enzymes, which include superoxide dismutase (SOD). This study aimed to assess the quality of semen from Itik Pinas-Khaki (IP-Khaki) drakes that were cryopreserved with either 4.5% DMSO or 7.0% glycerol as cryoprotectant through evaluation of total sperm motility (%) and determination of SOD activity (U/mL). Here, semen samples were collected from 12 sexually mature IP-Khaki drakes, an improved egg-type breed of Philippine mallard duck, and processed using modified reported cryopreservation procedure for ducks. Results showed that post-thawing total sperm motility averages of 12.04±5.61% using 4.5% DMSO and 13.99±5.28% using 7.0% glycerol were comparable. Moreover, similar SOD activity levels of 0.39±0.18 U/mL with 4.5% DMSO and 0.33±0.21 U/mL with 7.0% glycerol in 2.00 x 108 IP-Khaki sperm cells were also observed. The observed very low intracellular SOD activity indicates severe damage to sperm cells due to cryopreservation, which resulted in a comparably low total sperm motility with either of the cryoprotectants. Thus, the cryopreservation protocol used is not the optimum for IP-Khaki semen based on the observed considerable decline in sperm motility and very low SOD activity after cryopreservation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Superoxide Dismutase (SOD) Activity in Cryopreserved Semen of Itik Pinas-Khaki (Anas platyrhynchos L.)

Ancuelo

Landicho

Dichoso

et al. 2021

Trop. Anim. Sci. J.

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“…In total, 45 semen samples were collected over a period of 3 days from the 15 males. Ejaculates (n = 15/day) were immediately diluted with Beltsville poultry semen extender 1:1 (v:v) comprising potassium diphosphate (1.27 g), sodium glutamate monohydrate (0.867 g), fructose (0.5 g), sodium acetate anhydrous (0.43 g), Tris (hydroxymethyl)-aminomethane (0.195 g), potassium citrate monohydrate (0.064 g), potassium monophosphate (0.065 g), magnesium chloride (0.034 g), and H 2 O (100 ml); pH = 7.5, and osmolality 333 mOsm/kg 38 . Diluted semen samples were initially examined under a light microscope to ensure good quality of sperm (vigorous motility) and then kept in a water bath at 37 °C until use within half an hour of collection.…”

Section: Methodsmentioning

confidence: 99%

New insights into sperm rheotaxis, agglutination and bundle formation in Sharkasi chickens based on an in vitro study

El-Sherry

Abd‐Elhafeez

Sayed

2022

Sci Rep

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Fertility in birds is dependent on their ability to store adequate populations of viable sperm for extended durations in sperm storage tubules (SSTs). The exact mechanisms by which sperm enter, reside, and egress from the SSTs are still controversial. Sharkasi chicken sperm showed a high tendency to agglutinate, forming motile thread-like bundles comprising many cells. Since it is difficult to observe sperm motility and behavior inside the opaque oviduct, we employed a microfluidic device with a microchannel cross-section resembling close to that of sperm glands allowing for the study of sperm agglutination and motility behavior. This study discusses how sperm bundles are formed, how they move, and what role they may have in extending sperm residency inside the SSTs. We investigated sperm velocity and rheotaxis behavior when a fluid flow was generated inside a microfluidic channel by hydrostatic pressure (flow velocity = 33 µm/s). Spermatozoa tended to swim against the flow (positive rheotaxis) and sperm bundles had significantly lower velocity compared to lonesome sperm. Sperm bundles were observed to swim in a spiral-like motion and to grow in length and thickness as more lonesome sperm are recruited. Sperm bundles were observed approaching and adhering to the sidewalls of the microfluidic channels to avoid being swept with fluid flow velocity > 33 µm/s. Scanning and transmission electron microscopy revealed that sperm bundles were supported by a copious dense substance. The findings show the distinct motility of Sharkasi chicken sperm, as well as sperm's capacity to agglutinate and form motile bundles, which provides a better understanding of long-term sperm storage in the SSTs.

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“…Beberapa peneliti telah mencoba menggunakan bahan pengencer kimia maupun pengencer alternatif dari bahan-bahan alami. Pengencer yang umum digunakan dalam pengenceran semen ayam di Indonesia yaitu ringer laktat atau air kelapa yang disuplementasi kuning telur, karbohidrat dan antioksidan (Octa et al, 2014;Magfira et al, 2017;Telnoni et al, 2017, Iswati et al, 2017Malik et al, 2018;Khaeruddin dan Srimaharani, 2019; Khaeruddin dan Kurniawan, 2020). Pada pengencer semen ayam dibutuhkan adanya gula karena kurangnya komponen gula fruktosa yang terdapat secara alami dalam semen ayam yaitu 4 mg/100 ml semen (Getachew, 2016).…”

Section: Pendahuluanunclassified

Karakteristik Semen Segar dan Motilitas Spermatozoa Ayam Hutan Merah (Gallus Gallus) dalam Pengencer yang Mengandung Air Tebu

Arsyad

Kurniawan

Khaeruddin

et al. 2021

TROLIJA

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Penelitian ini bertujuan untuk mengetahui level air tebu terbaik untuk ditambahkan dalam pengencer semen ayam hutan merah berbasis ringer laktat kuning telur. Semen dikoleksi menggunakan metode pemijatan dan semen segar dievaluasi secara makroskopis dan mikroskopis. Semen dibagi ke dalam lima tabung yang masing-masing berisi pengencer ringer laktat kuning telur , ringer laktat kuning telur air tebu 10% , ringer laktat kuning telur air tebu 20% , ringer laktat kuning telur air tebu 30% , ringer laktat kuning telur air tebu 40% . Semen disimpan dalam refrigerator selama 24 jam kemudian dilakukan evaluasi motilitas spermatozoa (progresif, reverse, vibrator, total). Hasil penelitian menunjukkan bahwa perlakuan level air tebu dalam pengencer berpengaruh sangat nyata (P<0.01) terhadap motilitas progresif, motilitas reverse dan motilitas total. Penambahan level air tebu 10% dan 20% menghasilkan motilitas total paling tinggi yaitu 86.24-88.79%. Namun, motilitas progresif tertinggi didapatkan pada penggunaan level air tebu 20% yaitu 77.67%. Kesimpulan penelitian ini adalah air tebu 20% dapat ditambahkan dalam pengencer ringer laktat kuning telur untuk mempertahankan motilitas total dan motilitas progresif spermatozoa ayam hutan merah selama penyimpanan 24 jam.

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SK Kedu Semen Cryopreservation in Beltsville Poultry Semen Extender and Lactated Ringer’s-Egg Yolk Extender Using Dimethyl Sulfoxide

Cited by 8 publications

References 15 publications

Superoxide Dismutase (SOD) Activity in Cryopreserved Semen of Itik Pinas-Khaki (Anas platyrhynchos L.)

Superoxide Dismutase (SOD) Activity in Cryopreserved Semen of Itik Pinas-Khaki (Anas platyrhynchos L.)

New insights into sperm rheotaxis, agglutination and bundle formation in Sharkasi chickens based on an in vitro study

Karakteristik Semen Segar dan Motilitas Spermatozoa Ayam Hutan Merah (Gallus Gallus) dalam Pengencer yang Mengandung Air Tebu

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