A novel
approach for the in situ synthesis of
conjugated polyaniline-poly(ethylene glycol) graft copolymer (PA-g-PEG) by the combination of oxidative polymerization and
copper catalyzed azide–alkyne cycloaddition (CuAAC) click reaction
is described. The method pertains to the reduction of the CuBr2 catalyst during the oxidative copolymerization of aniline
and aminophenyl propargylether to Cu(I) species, which catalyze the
CuAAC reaction between thus formed polyaniline with pendant alkyne
groups and independently prepared azide functional PEG in both simultaneous
and sequential manner. The obtained water-soluble PA-g-PEG was used for the construction of glucose biosensor by a simple
one-step approach. Combined electrostatic polyanion-polycation and
hydrogen bond interactions between PA-g-PEG and glucose
oxidase provided a suitable immobilization matrix for the enzyme resulting
in excellent analytical parameters. PA-g-PEG based
glucose biosensor exhibited a remarkable response time, producing
an instant signal upon addition of analyte, making this sensor an
attractive alternative for the existing devices.
First and foremost, I must thank my supervisors Trine Grønhaug Halvorsen and Leon Reubsaet for helping me every step of the way and helping me to grow as a person and a scientist. I am especially grateful for their patience, understanding, and optimism. I am proud to be their Ph.D. student. I would be remiss not to thank my amazing collaborators Peter Cormack and Cem Biçak from the University of Strathclyde, Glasgow. Thank you for producing the materials used in this Ph.D. and for helping me to understand the complexities of imprinted polymers. To my masters' students, Frida Braathen and Jasna Petrovic, thank you for your invaluable contributions to this work. To all my colleagues throughout the years, thank you all so much for the amazing work environment, chats, and good times. You helped to create a wonderful work environment. I would like to especially thank Magnus Restan for helping me adjust to life in Norway and always being available for chats about life and football. To my family, I am thankful for your support and encouragement. I know it has been difficult with me being so far from home. Especially I'd like to thank Mark for sacrificing his Saturday mornings and helping with the graphical abstracts. Most of all I would like to thank my Zhenya. You have been amazing these last few years. Thank you so much for your love and support.
A fluorescent monomer-free one-step strategy is developed for the synthesis of fluorescent acrylamide gels, using inexpensive and commercially available rhodamine B as the hydrogen donor in type II photoinitiation system....
A new method for the synthesis of highly crosslinked and in situ alkyne functionalized microspheres, by combining type II photoinitiation with precipitation polymerization, is reported. Using a benzophenone‐tertiary amine initiation system, the method allows the preparation of functional microspheres at room temperature by UV irradiation in the absence of stabilizers or surfactants of any kind. To demonstrate the presence and accessibility of the alkyne moiety on the polymer particles, fluorescent pyrene groups are attached to the microspheres by copper(I)‐catalyzed alkyne‐azide cycloaddition click reaction, using 1‐azidomethyl pyrene as a model compound. FT‐IR, fluorescence spectroscopy, fluorescence microscopy, and scanning electron microscopy are used for characterization of the particles and to monitor the click reaction.
A simple and cost‐efficient method for fluorescent microsphere synthesis, which does not require any fluorescent monomers or modification steps to incorporate fluorescent moieties into the polymer particles, is reported. Using rhodamine B and benzophenone as bimolecular initiation system in type II photoinitiated precipitation polymerization, the method enables the preparation of fluorescent microspheres in one step, at room temperature and without the need for a stabilizer or surfactant of any type.
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