On-line duplex molecularly imprinted solid-phase extraction for analysis of low-abundant biomarkers in human serum by liquid chromatography-tandem mass spectrometry

The utility of molecularly imprinted polymers for mass spectrometric protein and proteomics analysis

“…Table 1 also contains information about the template used for imprinting. From the table it can be seen that the majority of the MIPs are produced using a template that is a modified version of the analyte, either a truncated form [51] or with protected or exchanged LOD [56,57] LOQ [57] Linearity [57] Precision [56] Yes [57] [ amino acids [26,[52][53][54][55][56][57]. This is an important precaution to ensure that residual template will not influence the determination of the target analyte.…”

“…However, for implementation of any of the methods in a routine setting, a more thorough validation is required. Additionally, some of the described methods need to be improved (e.g., improved LOD and precision around LOD [26], or improved accuracy [58]) to enable reliable protein quantification at relevant concentration levels in analysis of real sample.…”

“…In addition to the previously described formats, other more specialized formats are available. These are either for easy automation with LC-MS/MS through column switching [26,53,56,57] (all applied for tryptic peptide capture) or via a microfluidic platform with online reduction, alkylation, digestion and subsequent MIP enrichment [25] (applied in PTM capture of N-myristolated peptides), or modified MALDI plates with a MIP layer on top [23] (applied for intact protein capture). These specialized formats all have their separate advantages, but especially the microfluidic platform integrating reduction, alkylation, digestion and MIP enrichment may present a clear advantage over other formats.…”

“…of N-myristoylated peptides in human breast cancer cells [25], as well as on-line duplex analysis of two small-cell lung cancer biomarkers in serum digest [26].…”

Imidazole ionic liquid functionalized ZIF-67 molecularly imprinted solid-phase extraction coupled with high performance liquid chromatography for analysis of bisphenol A

“…The method of combining separation with molecularly imprinted materials and high performance liquid chromatography (HPLC) has been reported. 5 MIPs are a good solid-phase extraction agent, 6 lling material for the solid-phase extraction column 7 and the stationary phase of the HPLC column. 8 Bisphenol A (BPA) is a chemical, which is widely used in plastics, food packaging and medical devices (Fig.…”