Facilitating serum determination of neuron specific enolase at clinically relevant levels by coupling on-line molecularly imprinted solid-phase extraction to LC-MS/MS

Abstract

First and foremost, I must thank my supervisors Trine Grønhaug Halvorsen and Leon Reubsaet for helping me every step of the way and helping me to grow as a person and a scientist. I am especially grateful for their patience, understanding, and optimism. I am proud to be their Ph.D. student. I would be remiss not to thank my amazing collaborators Peter Cormack and Cem Biçak from the University of Strathclyde, Glasgow. Thank you for producing the materials used in this Ph.D. and for helping me to understand the complexities of imprinted polymers. To my masters' students, Frida Braathen and Jasna Petrovic, thank you for your invaluable contributions to this work. To all my colleagues throughout the years, thank you all so much for the amazing work environment, chats, and good times. You helped to create a wonderful work environment. I would like to especially thank Magnus Restan for helping me adjust to life in Norway and always being available for chats about life and football. To my family, I am thankful for your support and encouragement. I know it has been difficult with me being so far from home. Especially I'd like to thank Mark for sacrificing his Saturday mornings and helping with the graphical abstracts. Most of all I would like to thank my Zhenya. You have been amazing these last few years. Thank you so much for your love and support.

The utility of molecularly imprinted polymers for mass spectrometric protein and proteomics analysis

“…Table 1 also contains information about the template used for imprinting. From the table it can be seen that the majority of the MIPs are produced using a template that is a modified version of the analyte, either a truncated form [51] or with protected or exchanged LOD [56,57] LOQ [57] Linearity [57] Precision [56] Yes [57] [ amino acids [26,[52][53][54][55][56][57]. This is an important precaution to ensure that residual template will not influence the determination of the target analyte.…”

“…In addition to the previously described formats, other more specialized formats are available. These are either for easy automation with LC-MS/MS through column switching [26,53,56,57] (all applied for tryptic peptide capture) or via a microfluidic platform with online reduction, alkylation, digestion and subsequent MIP enrichment [25] (applied in PTM capture of N-myristolated peptides), or modified MALDI plates with a MIP layer on top [23] (applied for intact protein capture). These specialized formats all have their separate advantages, but especially the microfluidic platform integrating reduction, alkylation, digestion and MIP enrichment may present a clear advantage over other formats.…”

“…Common for all these formats is that they are performed off-line and easily can be adapted to the analysis technique available. Other formats such as the on-line formats [25,26,53,56,57] and the MIP layer on MALDI plate [23] are intended for use with a specific analytical technique. A schematic of an on-line microchip set-up is shown in Figure 6, and a schematic drawing of the MIP-MALDI plate can be found in Figure 7.…”

Porous Inorganic Materials for Bioanalysis and Diagnostic Applications

“…Three biomarkers (α-2-macroglobulin, complement C4-B, and α-1-antitrypsin) were significantly altered with the occurrence and progression of bladder cancer. Porous materials incorporated with downstream MS techniques have also been reported to play key roles in diagnosing pheochromocytoma, paraganglioma, serous ovarian cancer, peritoneal cancer, prostate cancer, and small cell lung cancer. , …”

“…Porous materials incorporated with downstream MS techniques have also been reported to play key roles in diagnosing pheochromocytoma, paraganglioma, serous ovarian cancer, peritoneal cancer, prostate cancer, and small cell lung cancer. 180,181 Other diseases have also been explored using the porous material-assisted MS approach. For instance, chronic unpredictable mild stress is a common complex psychiatric disorder with unclear pathophysiological mechanisms.…”