Bacillus megaterium T04 isolated from Rach Lang stream in Vietnam was tested for antimicrobial activities. The antimicrobial activities of Bacillus megaterium T04 (57.3x10 8 cfu/mL) against Candida albicans, Salmonella typhi, Pseudomonas aeruginosa, Staphylococcus sciuri, Micrococcus luteus were detected by agar well diffusion method in different cultivation conditions at three temperatures (25, 37, and 45oC) in three incubation periods (24, 48, and 72 hours). The efficacy of antimicrobial activities of this strain were determined in comparison with ceftriaxone activity against Candida albicans, Salmonella typhi, Pseudomonas aeruginosa, Staphylococcus scuiri, Micrococcus luteus. The antimicrobial activity potency was equivalent to ceftriaxone in a range ( 3.3 0.6 μg/mL to 46.5 6.2 μg/mL) for Candida albicans (0.9 0.2 μg/mL to 35.5 7.7 μg/mL) for Salmonella typhi, , (0.4 0.1 μg/mL to 28.4 4.4 μg/mL) for Pseudomonas aeruginosa, (119.8 21.2 μg/mL to 283.7 26.0 μg/mL) for Staphylococcus scuiri, (3.3 0.4 μg/mL to 64.4 7.4 μg/mL) for Micrococcus luteus.
Most of diseases are due to the "oxidative stress" resulting from an imbalance between formation and neutralization of pro-oxidants. Oxidant stress is initiated by free radicals, which produced aerobic metabolism in the body, can cause oxidative damage of biological macromolecules such as proteins, lipids, and DNA in healthy human cells (Yen and Chen, 1995; Gutteridge and Halliwell, 1993; Halliwell, 1995). These changes contribute to oxidative stress that is among the major causative factors in the induction of many chronic and degenerative diseases including atheorosclerosis, ischemic heart diseases and diabetes mellitus,
Phyllanthusurinariaplant was used in liver relating diseases in Vietnam. The study was to know more about the bioactivities of Phyllanthusurinarialeaf extracts before and after combined with Pandanustectoriousfruit extracts and Lactobacillus rhamnosusextract. This was accomplished by examining the influence of the total aqueous, chloroform and ethylacetateextracts. The biological tests were done onCandida albicans,Salmonella typhi, Pseudomonas aeruginosaand Staphylococcus aureus.Agar diffusion test was applied to determine the antimicrobial activities.Based on the inhibition zone diameter, the result showed that Phyllanthusurinariaaqueousextraction had the inhibition on the Pseudomonas aeruginosa(21.75 ± 0.96 mm)and Staphylococcus aureus(24.75 ± 0.96 mm). The activities decreased after combined with Pandanustectoriousfruit extractson Pseudomonas aeruginosa(14.75 ± 0.50 mm) and Staphylococcus aureus(14.75 ± 0.50 mm)and Lactobacillus rhamnosus(14.75 ± 0.50 mm).The total aqueous extracts fractionated with chloroform and ethylacetate still showed activities that suggested the polar and nonpolar antimicrobial compounds existed in Phyllanthusurinaria. However, the activities of these fractionated extracts in the combination showed the weaker activities than in the using only Phyllanthusurinariaextracts. Especially, there was no activity of the ethylacetate extracts in the combination with Lactobacillus rhamnosus. The study is the first report of the combination of Phyllanthusurinaria with Pandanustectorious and Lactobacillus rhamnosus PN04.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.