Antimicrobial Activities of Phyllanthus urinaria Extracts Before and After Combined with Pandanus tectorious and Lactobacillus rhamnosus PN04

Abstract

Phyllanthusurinariaplant was used in liver relating diseases in Vietnam. The study was to know more about the bioactivities of Phyllanthusurinarialeaf extracts before and after combined with Pandanustectoriousfruit extracts and Lactobacillus rhamnosusextract. This was accomplished by examining the influence of the total aqueous, chloroform and ethylacetateextracts. The biological tests were done onCandida albicans,Salmonella typhi, Pseudomonas aeruginosaand Staphylococcus aureus.Agar diffusion test was applied to determine the antimicrobial activities.Based on the inhibition zone diameter, the result showed that Phyllanthusurinariaaqueousextraction had the inhibition on the Pseudomonas aeruginosa(21.75 ± 0.96 mm)and Staphylococcus aureus(24.75 ± 0.96 mm). The activities decreased after combined with Pandanustectoriousfruit extractson Pseudomonas aeruginosa(14.75 ± 0.50 mm) and Staphylococcus aureus(14.75 ± 0.50 mm)and Lactobacillus rhamnosus(14.75 ± 0.50 mm).The total aqueous extracts fractionated with chloroform and ethylacetate still showed activities that suggested the polar and nonpolar antimicrobial compounds existed in Phyllanthusurinaria. However, the activities of these fractionated extracts in the combination showed the weaker activities than in the using only Phyllanthusurinariaextracts. Especially, there was no activity of the ethylacetate extracts in the combination with Lactobacillus rhamnosus. The study is the first report of the combination of Phyllanthusurinaria with Pandanustectorious and Lactobacillus rhamnosus PN04.