Essential oils were extracted from the culm and leaf of Cymbopogon citratus collected from different regions of Vietnam and analyzed using GC/MS. The results showed that citral is the major component accounting for 61.20%–76.46% of the essential oils. The citral content was higher in the essential oil obtained from the leaf than in that from the culm of lemongrass in all regions. In particular, camphene, valerianol, and epi-α-muurolol can be used to differentiate essential oils originating from leaves versus culms. The cytotoxic effects of the essential oils on various lung cancer cell lines were evaluated in the present study. All essential oils exhibited cytotoxicity in the tested cells. The Ha Loc leaf essential oil (HLL) exhibited the most potent effects on A549 and H1975 cells, with IC50 values of 1.73 ± 0.37 and 4.01 ± 0.30 μg/mL, respectively. The Hy Cuong leaf essential oil (HCL) showed the strongest effect on H1299 cells, with an IC50 value of 2.45 ± 0.21 μg/mL. The Kim Duc culm (KDC) essential oil exerted the strongest cytotoxic effects against H1650 cells, with an IC50 value of 4.86 ± 0.29 μg/mL. The HLL induced apoptosis and cycle arrest in A549 cells according to flow cytometric analysis and fluorescent nuclear staining assays. The western blot analysis indicated that HLL induced the apoptotic effect by altering the regulating proteins of the apoptosis process such as caspase-3, Bcl-2, and Bax. The data strongly suggested that the intrinsic pathway may play an important role in the apoptotic effects of HLL.

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Abstract. The river routing scheme (RRS) in the Organising Carbon and Hydrology in Dynamic Ecosystems (ORCHIDEE) land surface model is a valuable tool for closing the water cycle in a coupled environment and for validating the model performance. This study presents a revision of the RRS of the ORCHIDEE model that aims to benefit from the high-resolution topography provided by the Hydrological data and maps based on SHuttle Elevation Derivatives at multiple Scales (HydroSHEDS), which is processed to a resolution of approximately 1 km. Adapting a new algorithm to construct river networks, the new RRS in ORCHIDEE allows for the preservation of as much of the hydrological information from HydroSHEDS as the user requires. The evaluation focuses on 12 rivers of contrasting size and climate which contribute freshwater to the Mediterranean Sea. First, the numerical aspect of the new RRS is investigated, in order to identify the practical configuration offering the best trade-off between computational cost and simulation quality for ensuing validations. Second, the performance of the new scheme is evaluated against observations at both monthly and daily timescales. The new RRS satisfactorily captures the seasonal variability of river discharge, although important biases stem from the water budget simulated by the ORCHIDEE model. The results highlight that realistic streamflow simulations require accurate precipitation forcing data and a precise river catchment description over a wide range of scales, as permitted by the new RRS. Detailed analyses at the daily timescale show the promising performance of this high-resolution RRS with respect to replicating river flow variation at various frequencies. Furthermore, this RRS may also eventually be well adapted for further developments in the ORCHIDEE land surface model to assess anthropogenic impacts on river processes (e.g. damming for irrigation operation).

An ultrahigh-performance liquid chromatography in combination with high-resolution mass spectrometry Thermo Q-Extractive Focus Orbitrap MS has been introduced for analysis of multiclass pesticides in vegetable samples collected in Hanoi, Vietnam. Multiclass pesticides were separated on the Thermo Hypersil Gold PFP column utilizing a gradient of the mobile phase consisting of 5 mM ammonium formate, 0.1% formic acid in deionized water, and methanol. The target analytes were detected in the full-scan mode on Thermo Scientific Q-Exactive Focus Orbitrap MS for quantitation at the optimum operating conditions. These conditions included, but not limit to, the resolution of 70000 at the full width at half maximum in both positive and negative mode, mass range from 80 to 1000 m/z, and optimized parameters for the heated electrospray ionization source. The identification of the analytes in real samples was based on retention times, mass to charge ratios, mass accuracies, and MS/MS spectra at the confirmation mode with the inclusion list of target analytes. The mass accuracies of target analytes were from −4.14 ppm (dinotefuran) to 1.42 ppm (cinosulfuron) in the neat solvent and from −3.91 ppm (spinosad D) to 1.29 ppm (cinosulfuron) in the matrix-matched solution. Target analytes in the vegetable-based matrix were extracted by the QuEChERS method. Some critical parameters of the analytical method such as linearity, repeatability, limit of detection, and limit of quantitation have been evaluated and implemented. Excellent LOD and LOQ of the developed method were achieved at the range of 0.04–0.85 and 0.13–2.9 μg·kg−1, respectively. Intraday and interday repeatability of the analytical signal (peak area, n=6) of the developed method were below 3% and 10%, correspondingly. The matrix effect, extraction recovery, and overall recovery were fully investigated by spiking experiments. Experimental results demonstrated that the ionization suppression or enhancement was the main contribution on the overall recoveries of target analytes. Finally, the in-house validated method was applied to pesticides screening in vegetables samples in local villages in Hanoi, Vietnam. The concentrations of all target analytes were below limit of quantitation and lower than US-FDA or EU maximum residue levels.

e mud crab increases the yield of farming in Southeast Asian countries. Scylla paramamosain, one of four mud crab species belonging to the Scylla genus, is a rich nutrient source during its soft-shell moulting period. In this study, we analysed the total lipid content, fatty acid components, and phospholipid molecular species of the S. paramamosain mud crab. e total lipid content was 1.62 ± 0.08%, which is similar to that of S. serrata previously reported. Twenty-one fatty acids were identified in S. paramamosain. e composition and molecular forms of the phospholipids were identified using high-performance liquid chromatography-high-resolution mass spectrometry. Fifty-four different molecules belonging to six types of phospholipids were identified. Notably, phospholipids were made of fatty acids with C16 : 0; C18 : 0; C20 : 4; C20 : 5; and C22 : 6 main components. e anti-inflammatory and cytotoxic effects of crab lipids and phospholipids were investigated for the first time.e anti-inflammatory activity of the total and polar lipids had IC 50 values of 71.5 and 68.6 μg/mL, respectively. e crab polar lipid fraction, which contained phospholipids, also presented high cytotoxic activity toward five cancer cell lines with IC 50 values ranging from 85.4 to 95.8 μg/mL.