Fatty Acid Composition, Phospholipid Molecules, and Bioactivities of Lipids of the Mud Crab Scylla paramamosain

Abstract

e mud crab increases the yield of farming in Southeast Asian countries. Scylla paramamosain, one of four mud crab species belonging to the Scylla genus, is a rich nutrient source during its soft-shell moulting period. In this study, we analysed the total lipid content, fatty acid components, and phospholipid molecular species of the S. paramamosain mud crab. e total lipid content was 1.62 ± 0.08%, which is similar to that of S. serrata previously reported. Twenty-one fatty acids were identified in S. paramamosain. e composition and molecular forms of the phospholipids were identified using high-performance liquid chromatography-high-resolution mass spectrometry. Fifty-four different molecules belonging to six types of phospholipids were identified. Notably, phospholipids were made of fatty acids with C16 : 0; C18 : 0; C20 : 4; C20 : 5; and C22 : 6 main components. e anti-inflammatory and cytotoxic effects of crab lipids and phospholipids were investigated for the first time.e anti-inflammatory activity of the total and polar lipids had IC 50 values of 71.5 and 68.6 μg/mL, respectively. e crab polar lipid fraction, which contained phospholipids, also presented high cytotoxic activity toward five cancer cell lines with IC 50 values ranging from 85.4 to 95.8 μg/mL.

Lipidomics and Anti-Inflammation Activity of Brown Algae, Lobophora sp., in Vietnam

“…Recent researches on lipid usually have applied GC-MS, GC-FID techniques to detect the composition and content of fatty acids; however, fatty acids exist in both free and linked types. Nowadays, the application of HR-MS technique helps identify the hold of lipid molecules and even molecular species based on ion values of MS [17,18,21]. Some novel techniques such as LC-MS/MS have been applied to study the lipidome of seaweed [9,10,14,15,37].…”

“…Total lipid was extracted according to method of Bligh and Dyer [16] and Nguyen et al [17]. Polar lipids were obtained by the silica column.…”

“…e image of thin layer was obtained using Epson Perfection 2400 scanner, Japan, with grayscale mode. e lipid layers on the thin plate and the percentage of the layers were identified by light sensitivity on the Sorbfil TLC Analysis software Video densitometer, Krasnodar, Russia [17,18].…”

“…Temperature program in the oven operating was at 160°C, raised 2°C/min to 240°C, and then kept for 20 minutes. Fatty acids were identified by the equivalent retention time value, Equivalent Chain Length, with the standard system of fatty acids C16 : 0 and C18 : 0. e fatty acid structures were identified by GC-MS. e spectra were compared with the NIST library and fatty acid mass spectra archive [17,18].…”

“…e polar lipid classes were separated by HPLC in a solvent A: n-hexane/2-propanol/formic acid/(C 2 H 5 ) 3 N and the solvent B: 2-propanol/H 2 O/formic acid/(C 2 H 5 ) 3 N [19] and then detected by the high-resolution ion trap over time of mass spectrometry using the LC/MS-IT-TOF (Shimadzu) device, an electronic atomizing ionization source (ESI) device. Flow rate was 0.2 ml/min [17,20,21]. Assay. In vitro antiinflammatory activity of lipid samples was elucidated by using nitric oxide (NO) assay as described previously by Mosmann [22] using the Griess reagents (Promega, USA).…”