LLLT at 670 nm positively influences all phases of rat skin wound healing.

Vidinsk˘ B., P. Gal, T. Toporcer, F. Longauer, L. Lenhardt, N. Bobrov, J. Sabo: Histological Study of the First Seven Days of Skin Wound Healing in Rats. Acta Vet. Brno 2006, 75: 197-202.The aim of this study was to elaborate a histological model of incisional skin wound healing in Sprague-Dawley rats. Under aseptic conditions two paravertebral full thickness skin incisions were performed on the back of 42 anesthetized male rats. Histological sections from tissue specimens were stained by hematoxylin and eosin, van Gieson, PAS + PSD, Mallory's phosphotungstic hematoxylin and azur and eosin and evaluated during the first seven days after surgery. Histological evaluation revealed that the regeneration of injured epidermis was completed five days after surgery. The inflammatory phase was recorded during the first three days of healing with the culmination of this phase between day one and day two. The beginning of the proliferative phase was dated to the first day and the peak during day five and day six. The initiation of the maturation and remodeling phase of the healing process was observed six days after wounding. At the layer of striated muscle, the centronucleated cells were described for the first time six days after surgery. The wound healing process of rat skin was histologically described during the first seven days. Results of this work can serve as an experimental model for further research using external pharmacological and physical factors (laser light, magnetic field) by which the wound healing can be favourably influenced.Sprague-Dawley rats, inflammatory phase, proliferative phase, maturation in remodeling phase, histology

The effect of Atropa belladonna L. (AB) aqueous extract on skin wound healing was studied in male Sprague-Dawley rats subjected to two parallel full-thickness skin incisions on the back. Specimens for histological evaluation were collected on days 2 and 5 whereas for biomechanical testing, they were collected on day 5. In the in vitro study, a different concentration of AB extract was used to test the differentiation of keratinocytes using a panel of selected antibodies, proliferation, and cell survival of 3T3 fibroblasts and human umbilical vein endothelial cells using the MTT-assay. Results of the in vivo experiments showed in AB-treated wounds a shortened process of inflammation and accelerated collagen formation, as well as significantly increased wound stiffness as compared with control tissues. The in vitro examination showed that control keratinocytes were cytokeratin 19 free, while samples exposed to the highest AB extract concentration expressed CK19. Moreover, all concentrations were stimulatory to human umbilical vein endothelial cell proliferation. In addition, only the AB extract at the lowest tested concentration increased fibroblast growth, but higher concentrations decreased cell survival. In conclusion, our results indicate that the AB water extract positively affects early phases of skin wound healing in rats. However, the in vitro results on the inverse relation between the concentration of the AB extract and its effects on cell proliferation may be important for future research.

Medzi najčastejšie primárne nádory pulmonálnej chlopne a pulmonálnej artérie patrí schwanóm, myxóm, papilárny fi broelastóm, primárny choriokarcinóm a sarkóm. Výskyt týchto histologických typov nádorov je veľmi raritný. Medzi najčastejšie klinické manifestácie tumorov pulmonálnej artérie patrí dýchavica, bolesť na hrudi, kašeľ a hemoptýza. Prezentovaná je kazuistika 44-ročného muža s anamnézou zhoršujúcej sa dýchavice, febrilít, kašľa a straty hmotnosti. Vyšetrenia zobrazovacími metódami poukázali na veľkú nasadajúcu embóliu výtokovej časti pravej komory, pulmonálnej chlopne a kmeňa pulmonálnej artérie. Pacient bol prijatý do nemocnice za účelom antikoagulačnej terapie. Vzhľadom na neúspešnosť konzervatívnej terapie bol pacient indikovaný k operácii. Peropečne bol zistený tumor pulmonálnej chlopne vypĺňajúci takmer celý lumen pulmonálnej artérie a viaceré menšie tumory výtokového traktu pravej komory a pulmonálnej artérie. Histologické vyšetrenie tumoru poukázalo na leiomysarkóm high grade 3. Prezentovaná kazuistika zdôrazňuje, že tumor pulmonálnej artérie by mal byť stále zahrnutý do diferenciálnej diagnostiky pulmonálnej embólie. To platí najmä v prípade zvýraznenia symptómov i pri adekvátnej antikoagulácii a absencii rizikových faktorov hlbokej žilovej trombózy. Vzhľadom na neskorú diagnostiku ostáva prognóza pacientov so sarkómom pulmonálnej chlopne hrozivá.

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