Third degree burn is a special wound cause consist of eschar for a long time. Kept eschar in third degree burn will delay healing procces. That is why eschar should be disapper by debridement process. Mechanical debridement used in this research. The aim of this research is to know the differences between mechanical debridement 24 hour and 48 hour in healing acceleration. This research is a true experimental by using post test only control group design. The samples was selected by simple random sampling and was divided into three groups, each group consist of six rats, they are 24 hour group, 48 hour group and control group. The data was analyzed by one-way ANOVA then followed by Post Hoc LSD. The one way ANOVA result p = 0,018 (p < 0,05) so there was a significant difference between control group and experimental groups. But the Post Hoc LSD results p = 0,123 ( p > 0,05) so there was no significant difference between 24 hour group and 48 hour group in healing acceleration. For further researchers, it is suggested to use microscopic technique to know the effect of mechanical debridement in healing acceleration. ABSTRAKLuka bakar derajat III merupakan luka yang unik karena terdapat sejumlah besar eskar yang tetap berada pada tempatnya dalam waktu yang lama. Eskar yang tetap dipertahankan akan menghambat penyembuhan luka. Oleh karena itu eskar harus dihilangkan melalui proses debridemen. Penelitian ini bertujuan untuk mengetahui perbedaan kecepatan penyembuhan luka antara debridemen mekanik 24 jam dan 48 jam paska luka bakar derajat III. Penelitian ini merupakan penelitian murni (true eksperimental) dengan rancangan post test only control group design. Sampel dipilih secara simple random sampling yang dibagi dalam 3 kelompok masing-masing 6 ekor tikus, yaitu kelompok 24 jam, kelompok 48 jam dan kelompok kontrol. Uji statistik yang digunakan adalah one way ANOVA kemudian dilanjutkan uji Post Hoc LSD. Hasil uji one way ANOVA nilai p = 0,018 (p < 0,05) sehingga dapat disimpulkan terdapat perbedaan signifikan rata-rata lama penyembuhan luka pada ketiga kelompok.
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