GaN-based light-emitting diode (LED) has been widely used in recent years, and tremendous progress has been achieved in GaN-based semiconductor materials and relevant process. However, owing to the large refractive index contrast between GaN-based semiconductor materials and air, light can be easily totally internally reflected at the semiconductor/air interface, and the critical angle for light to escape from the semiconductor is small. Therefore, the light extraction efficiency for GaN-based LED is still low and needs improving. Some of the leading approaches to enhance light extraction efficiency of GaN-based LED such as surface texturing or roughening, omnidirectional reflectors, photonic crystals, laser liftoff, transparent electrode, patterned substrate and so on are introduced in detail. For each approach, how the variation in device structure or material improves the light extraction efficiency is analyzed thoroughly. At last, some of mentioned approaches that are promising are evaluated and viewed briefly.
Introductionβ-Mannanases can hydrolyze mannans, which are widely available in nature. However, the optimum temperature of most β-mannanases is too low to be directly utilized in industry.MethodsTo further improve the thermostability of Anman (mannanase from Aspergillus niger CBS513.88), B-factor and Gibbs unfolding free energy change were used to modify the flexible of Anman, and then combined with multiple sequence alignment and consensus mutation to generate an excellent mutant. At last, we analyzed the intermolecular forces between Anman and the mutant by molecular dynamics simulation.ResultsThe thermostability of combined mutant mut5 (E15C/S65P/A84P/A195P/T298P) was increased by 70% than the wild-type Amman at 70°C, and the melting temperature (Tm) and half-life (t1/2) values were increased by 2°C and 7.8-folds, respectively. Molecular dynamics simulation showed reduced flexibility and additional chemical bonds in the region near the mutation site.DiscussionThese results indicate that we obtained a Anman mutant that is more suitable for industrial application, and they also confirm that a combination of rational and semi-rational techniques is helpful for screening mutant sites.
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