A novel bilayer knitted fabric-reinforced composite for potentially being used as a dural substitute was developed by solution infiltration of oxidized regenerated cellulose knitted fabric (ORC) with poly ε-caprolactone (PCL) solution at various concentrations ranging 10-40 g/100 mL. It was found that the density of all formulations did not differ significantly and was lower than that of the human dura. Microstructure of the samples typically comprised a bilayer structure having a nonporous PCL layer on one side and the ORC/PCL composite layer on another side. Tensile modulus and strength of the samples initially decreased with increasing PCL solution concentration for up to 20 g/100 mL and re-increased again with further increasing PCL solution concentration. Strain at break of all formulations were not significantly different. Watertight test revealed that all composites could prevent leakage at the pressure within the normal range of intracranial pressure. In vitro degradation study revealed that the weight loss percentage and change in tensile properties of all samples displayed biphasic profile comprising an initially rapid decrease and followed by a gradual decrease with incubation times afterward. Micro and macro porous channels were observed to be in situ generated in the composite layer by ORC dissolution and PCL resorption during degradation while nonporous layer remained relatively unchanged. The degradation rate was found to decrease with increasing PCL solution concentration. In vitro biocompatibility using alamar blue assay on selected samples showed that fibroblasts could attach and proliferate well at all incubation periods.
An inadequate dural closure is one of the most challenging problems for neurosurgeons during the surgical procedures. A repair of the dura mater by natural or synthetic materials is often needed. This should satisfy fundamental criteria for example preventing cerebrospinal fluid leakage, exhibiting similar mechanical properties to the natural dura mater and not inducing foreign body reaction or inflammation. Oxidized regenerated cellulose (ORC) and polycaprolactone (PCL) have been extensively used as hemostatic agent and implant in many biomedical applications due to their long term proven safety, biodegradability and biocompatibility. This study investigated the potential of using a combination of ORC and PCL as a novel dural substitute. ORC/PCL composites were prepared by solution infiltration of ORC sheet with PCL solution (Mw ≈ 80,000) at various concentrations ranging 10-50 g/100 ml. Characterizations including density, tensile properties and microstructure were then performed. It was found that the density of all formulations did not differ and were in the range of 0.5-0.6 kg m-3. Microstructure of the samples typically comprised a bilayer structure having a PCL layer on one side and the ORC/PCL mixed layer on another side. Tensile modulus and strength initially decreased with increasing PCL concentration for up to 20% and re-increased again with further increasing PCL concentration. Elongation at break of all formulations was not significantly different. Both physical and mechanical properties of the samples were found to be similar to those of natural human dura mater.
In recent years, a lot of attention has been focused on using adipose‐derived mesenchymal stem cells obtained from infrapatellar fat pad (IPFP‐ASCs) for the articular cartilage regeneration. IPFP‐ASCs constructs were previously characterized and demonstrated chondrogenic differentiation potential to produce hyaline like‐cartilage in vitro. However, little is known about the relationship of its regeneration potential and pain associated with osteochondral defect. This study aimed to investigate the effect of implantation of the 3‐Dimensional (3D) cartilage construct of IPFP‐ASCs on the restoration of an articular hyaline cartilage as well as attenuation of pain associated with the cartilage defect in an osteochondral defect rat model. The chondrogenic differentiation potential of the 3D cartilage construct derived from IPFP‐ASCs was determined prior to implantation and at 4, 8 and 12 weeks post‐implantation by gene expression and immunochemistry analysis. Pain‐related behavior was examined weekly up to 8 weeks post‐implantation by using weight‐bearing test. A significant pain‐associated with osteochondral defect was observed in this model in all groups post‐induction; however, this pain can spontaneously resolve within three weeks post‐implantation regardless of implantation of IPFP‐ASCs constructs. The existences of mature chondrocytes as well as a significant (p<0.05) positively immunostained for type II collagen and aggrecan were identified in the implanted site for up to 12 weeks compared to untreated group, indicating the hyaline cartilage regeneration. Overall, this study reported the successful outcome of osteochondral regeneration with scaffold‐free IPFP‐ASCs constructs in an osteochondral defect rat model. Although the implantation of the cartilage construct could not attenuate pain associated with the cartilage defect, it provides novel and interesting insights into the current hypothesis that 3D construct IPFP‐ASCs may have potential benefits as an alternative approach to repair bone and cartilage defect.
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