Asian soybean rust, caused by the fungus Phakopsora pachyrhizi, is one of the most important diseases affecting soybean production in tropical areas. During infection, P. pachyrhizi secretes proteins from haustoria that are transferred into plant cells to promote virulence. To date, only one candidate P. pachyrhizi effector protein has been characterized in detail to understand the mechanism by which it suppresses plant defenses to enhance infection. Here, we aimed to extend understanding of the pathogenic mechanisms of P. pachyrhizi based on the discovery of host proteins that interact with the effector candidate Phapa-7431740. We demonstrated that Phapa-7431740 suppresses pathogen-associated molecular pattern triggered immunity (PTI), and it interacts with a soybean glucan endo-1,3- β-glucosidase (GmβGLU), a pathogenesis-related (PR) protein belonging to the PR-2 family. Structural and phylogenetic characterization of PR-2 protein family predicted in the soybean genome and comparison to PR-2 family members in Arabidopsis thaliana and cotton, demonstrated that GmβGLU is a type IV β-1,3-glucanase. Transcriptional profiling during an infection time course showed that the GmβGLU mRNA is highly induced during the initial hours after infection, coinciding with peak of expression of Phapa-7431740. The effector was able to interfere with the activity of GmβGLU in vitro, with a dose-dependent inhibition. Our results suggest that Phapa-7431740 may suppress PTI by interfering with glucan endo-1,3-β-glucosidase activity.
RESUMO A mancha bacteriana do tomateiro, causada por quatro espécies de Xanthomonas pode provocar perdas significativas na produção da cultura e a utilização de biofertilizantes na proteção de plantas tende a reduzir a incidência de doenças. O objetivo do trabalho foi avaliar o efeito dos biofertilizantes no controle preventivo e curativo da mancha bacteriana do tomateiro. Para o controle preventivo da doença, plantas de tomate cultivar Santa Cruz Kada, com 3 a 4 folhas foram pulverizadas com os biofertilizantes (Soil-Set, Agro-Mos e Cop-R-Quick) e água (testemunha); e dois dias após foram inoculadas por aspersão com a suspensão bacteriana nas concentrações 109 UFC mL-1 (OD550=0,5) e 106UFC mL-1, com o isolado UFU A35 de Xanthomonas sp. Para o controle curativo, as plantas foram inoculadas com a suspensão bacteriana, e dois dias após foram pulverizadas com os biofertilizantes e água. A severidade da mancha bacteriana foi avaliada usando uma escala diagramática; aos 3, 5, 8, 11 e 14 dias após a inoculação e calculada a área abaixo da curva de progresso de doença (AACPD). O controle preventivo foi mais eficiente no manejo da mancha bacteriana do tomateiro, e os diferentes biofertilizantes reduziram a severidade da doença.
Conservation of vegetative organs, as leaves and stalks, is essential to achieve high maize production. The objective of this work was to evaluate the effects of leaf area maintenance due to fungicides spraying on stalk integrity, its chemical composition and grain yield. The experiment was conducted in Uberlândia - MG, during the second season of 2016. The experiment design was a randomized complete block with six replications and six treatments, representing combinations of fungicides belonging to carboxamide, strobilurin, triazole, and dithiocarbamate chemical groups, and the check treatment (without fungicides). The assessed traits were leaf green area, wet and dry stalk density, stalk breaking strength and height, stalk lignin and cellulose percentage, stalk dry matter, stalk lignin and cellulose content per hectare and grain yield. Treatments containing fungicides, especially the ones containing carboxamide, resulted in higher leaf green area and stalk density. Application of fungicides also increased stalk breaking strength and height; however, no effect was observed on stalk lignin and cellulose percentage. Grain yield, stalk dry matter production, and stalk lignin and cellulose contents per hectare were enhanced by fungicide application, with higher improvements in plants treated with carboxamide. Application of fungicides, mainly when combined with carboxamides, promotes higher maintenance of leaf green area, which leads to improved stalk integrity, higher grain yield, and higher straw production.
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