Objectives The aim of this study was to evaluate the degree of conversion and bond strength of a commercial dental adhesive modified by the incorporation of quercetin, resveratrol (RES), and Brazilian red propolis (BRP). Methods BRP markers were identified using ultra-performance liquid chromatography coupled with a diode array detector, and the antioxidant activity (AAO) of the three substances was analyzed. Single Bond 2 adhesive (3M ESPE) was modified by adding BRP, quercetin, and RES, separately, at 20 μg/mL, 250 μg/mL, and 500 μg/mL, respectively. The degree of conversion (DC) was measured using near-infrared spectroscopy 24 h after photopolymerization. Measurements of the resin-dentin microtensile bond strength (μTBS) were carried out after 1 day and 1 year. Student's t test and ANOVA with Tukey's test were used for data analysis (α = 0.05). Results The markers daidzein, liquiritigenin, pinobanksin, isoliquiritigenin, formononetin, pinocembrin, and biochanin A were found in the ethanolic extract of BRP. Quercetin, RES, and BRP showed high AAO. The DC of the tested adhesives remained adequate for this category of material, with a slight increase in the DC of adhesives with quercetin and BRP ( P > 0.05). Comparisons between μTBS measurements made at 1 day and 1 year showed that, contrary to the control group, μTBS values for all modified adhesives were maintained after 1 year in distilled water ( P > 0.05). Conclusions These findings suggest that quercetin, RES, or BRP might be useful in adhesive dentistry to help improve hybrid layer resistance. Clinical significance Dentin bonding agents with quercetin, RES, and BRP have potential to increase the longevity of composite restorations.

O Papiloma Vírus Humano (HPV) é um vírus de DNA, pertencente à família Papillomaviridae, o qual possui potencial oncogênico, sendo responsável por aproximadamente 98% dos casos de câncer do colo do útero. Esse tipo de câncer é a principal causa de morte entre mulheres que vivem em países em desenvolvimento. O Instituto Nacional de Câncer (INCA) estima que para o ano de 2019 sejam diagnosticados 16.370 novos casos de câncer de colo do útero no Brasil, com um risco estimado de 15,43 casos a cada 100 mil mulheres. Com base no exposto, objetivou-se realizar uma revisão literária sobre o HPV e sua importância na indução de neoplasias cervicais, a fim de descrever e evidenciar as vantagens do uso da vacina de proteção contra este vírus. As vacinas anti-HPV foram criadas com o intuito de prevenir a infecção pelo vírus e, dessa forma, reduzir o desenvolvimento de neoplasias cervicais. Estas vacinas contêm uma proteína capsídeo L1 do HPV que se autorreproduz em partículas virus-like (VLP) capazes de neutralizar as infecções naturais subsequentes. Atualmente, encontram-se disponíveis no mercado duas vacinas profiláticas regulamentadas para uso na população em diversos países, incluindo o Brasil: a vacina bivalente Cervarix® que atua contra os vírus 16 e 18 e a vacina tetravalente Gardasil® que atua contra os sorotipos 6,11, 16 e 18. Entre as informações mais importantes, destacam-se que está indicada para mulheres entre 9 e 26 anos e meninos entre 11 e 14 anos, antes da iniciação sexual, sendo administrada em três doses; possui eficácia comprovada contra os sorotipos nela presentes, sendo altamente imunogênica e com garantia de proteção por cerca de 10 anos.

Despite the huge improvements made in adhesive technology over the past 50 years, there are still some unresolved issues regarding the durability of the adhesive interface. A complete sealing of the interface between the resin and the dentin substrate remains difficult to achieve, and it is doubtful whether an optimal interdiffusion of the adhesive system within the demineralized collagen framework can be produced in a complete and homogeneous way. In fact, it is suggested that hydrolytic degradation, combined with the action of dentin matrix enzymes, destabilizes the tooth-adhesive bond and disrupts the unprotected collagen fibrils. While a sufficient resin–dentin adhesion is usually achieved immediately, bonding efficiency declines over time. Thus, here, a review will be carried out through a bibliographic survey of scientific articles published in the last few years to present strategies that have been proposed to improve and/or develop new adhesive systems that can help prevent degradation at the adhesive interface. It will specially focus on new clinical techniques or new materials with characteristics that contribute to increasing the durability of adhesive restorations and avoiding the recurrent replacement restorative cycle and the consequent increase in damage to the tooth.

Methicillin resistance in the Staphylococcus intermedius group (SIG) has emerged in small animal practice. Methicillin-resistant SIG (MRSIG) members have been implicated as causes of infections in both companion animals and humans. Staphylococcal cassette chromosome mec (SCCmec) elements carry the mecA/C genes, which encode for the transpeptidase PBP2a (PBP2’) responsible for β-lactam antibiotic resistance in staphylococci. This study examined the SCCmec types of MRSIG isolates from different clinical specimens of dogs that exhibited methicillin MIC ≥ 0.5 μg/mL by an automated identification and susceptibility system in a Center for Veterinary Diagnostics in São Paulo, Brazil. Susceptibility to methicillin was determined by broth microdilution testing, and Oxoid® M.I.C.Evaluator® strips. PBP2a production was detected using a latex agglutination assay. SCCmec typing was performed according to the International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements (IWG-SCC) guidelines. SCCmec type II (2A), SCCmec type III (3A), composite SCC structures consisting of a class A mec gene complex in addition to multiple ccr gene complexes, and non-typable SCCmec elements were reported in these MRSIG isolates. SCCmec type variants differing from those so far acknowledged by IWG-SCC were found, indicating new rearrangements in the genetic context of mecA in these canine MRSIG isolates.