-Polymerase Chain Reaction (PCR) technology has been widely used in molecular biology, forensic analysis, evolutionary and medical diagnostic. PCR requirement for point of care application is shorten reaction time and reduces PCR reagent. If the PCR chip can be done by droplet basis, then the analysis system which minimal amount of fluid will be possible. In this report, we describe the simulation and experimental study of a parallel-plate electrowetting on dielectric (EWOD) based PCR device. The EWOD device is applied to control PCR reagent and sample for a polymerase chain reaction (PCR) system. The EWOD-based PCR system consists of 3.4 mm wide, 15.1 mm long serpentine-shape microheater integrated underneath to maintain temperature at each stage and 370 μm deep microchannel. DNA was driven by moving droplet through each square electrode (dimension 1x1 mm 2 ) that maintains temperature at denaturation, annealing, and extension region. The fluid dynamics of the device have been modeled by Coventorware , a commercially available MEMS simulation software, to show temperature distribution on each electrode. In experiment, this work studied contact angle reduction of different droplet because this result can be studied possibility of transporting of PCR reagent for a droplet based PCR.
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