Background: ESBL producing bacteria are increasing with an alarming rate with a wide range of infections. Objective: The purpose of the present study was to see the status of ESBL producing bacteria isolated from skin wounds. Methodology: This cross sectional study was conducted in the Department of Microbiology at Mymensingh Medical College, Bangladesh from January 2011 to June 2011 for a period of 6 months. All the patients, at any age with both sexes presented with skin wound infection, were taken as study population. Wound swab was taken from all patients. Specimens were processed and bacteria were isolated and identified according to standard procedure. The ESBL status was confirmed by double disc diffusion test (DDDT) and minimum inhibitory concentration (MIC) by agar dilution method by standard procedure according to Clinical Laboratory Standard Institute (CLSI). Antimicrobial resistance was done by disc diffusion method. Result: A total number of 84 wound swabs were taken of which the most common ESBL producing bacteria were Esch. coli (61.5%), Proteus species (78.3%) and Klebsiella species (88.9%). All the isolates were sensitive to imipenem and nitrofurantoin followed by amikacin (92.9%). Conclusion: In conclusion, ESBL producing E. coli is the most common bacteria causing skin wound infection followed by Proteus species with a reduced sensitivity towards antibiotics.
Background: Bacterial etiologies of febrile illnesses have significantly become important due to high mortality and morbidity. Objective: This study aimed to identify the prevalence of rickettsial fever, brucellosis, and typhoid fever by triple antigen test. Methodology: This cross-sectional study was conducted at TMSS Medical College Bogura district in Bangladesh for 6 months. Febrile patients aged from 1 to 65 years of both sexes were recruited from the outpatient department of this Medical College Bogura. Blood sample were taken from all patients for antibody test by microscopic plate agglutination test according to manufacturer instruction. Results: A total of 140 patients were enrolled and 74/140 (52.86%) patients were positive by any of three antigens, among them female were 54/74 (72.97%), most common age group was 21 to 30 years which was 22/74(29.73%), TO titre was greater than TH titre, which was 24/36 (66.66%). Conclusion: The study has underscored the importance of febrile antigen detection for bacterial diseases including zoonosis such as ricketial fever, brucellosis and typhoid fever in febrile patient and thus reducing diagnostic dilemma of febrile diseases. However, further diagnostic tests for diagnosis of febrile illnesses also needed. This will be helpful for febrile patients to receive the correct diagnoses and facilitation of accurate and prompt treatment. Bangladesh Journal of Infectious Diseases 2020;7(1):18-21
Extended spectrum beta lactamases (ESBLs) produced by Gram negative bacteria are mainly mediated by three important genes, namely TEM, SHV and CTX-M. In this study, we used a multiplex PCR to determine the prevalence of CTX-M and its subgroups CTX-M-3, CTX-M-14, among the members of Enterobacteriaceae family and in Pseudomonas spp that were isolated from different clinical samples in a tertiary care hospital in Bangladesh. A total of 300 culture positive clinical isolates were selected for the study. Out of these, 216 from urine, 45 from wound swab, 39 from pus aspirates. The ESBL status was determined by double disc diffusion test (DDDT) as recommended by Clinical Laboratory Standard Institute 2010 (CLSI) and by multiplex PCR for TEM, SHV and CTX-M, CTX-M-3, CTX-M-14 genes. Out of 300 isolates tested, 71.3% were positive for ESBL production by DDDT. The rate of positivity for TEM, SHV and CTX-M genes in 107 randomely selected isolates was 83.2%. Among these, 56.2% (50/89) was positive for CTX-M. Among the CTX-M positive isolates, CTX-M-3 and CTX-M- 14 were 78.0% (39/50) and 80.0% (40/50) respectively. Our study demonstrated that CTX-M variants were common in Enterobacteriaceae and Pseudomonas spp prevalent in the hospital of Bangladesh.Ibrahim Med. Coll. J. 2015; 9(1): 26-30
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