In order to identify the medicinal and aromatic plants most requested for the treatment of the most common oral pathology, an ethnobotanical survey was carried out in the economic capital Casablanca, Morocco. The data basis was obtained draw selected traditional herbalists based on the semi-structured questionnaire. Quantitative indices such as use value (UV), family UV (FUV), fidelity level and informant consensus factor (ICF) were intended to evaluate the importance of plant species. A total of 46 plants species belonging to 22 families that were used. Juglandaceae family showed the highest significance (FUV = 0.75). We identified 40 species used for gum disease (gingivitis, periodontal abscess), 15 for dental pain (toothache, tooth sensitivity), 14 for halitosis, 12 for oral ulcers (aphtous, mouth ulcers and herpes), 3 for dental stain (teeth cleaning, sparkling and bleaching) and only 2 for tooth decay. The used plants are mainly prepared as decoction (80.4%). Syzygium aromaticum (UV = 0.94) was the specie most commonly prescribed by local herbalists. The higher ICF (0.75) was registered for the use gum disease.Graphical Abstract Electronic supplementary materialThe online version of this article (10.1007/s13659-018-0194-6) contains supplementary material, which is available to authorized users.

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The bactericidal activity of a pulsed fibre-optic Nd-YAP laser on various strains of oral bacteria: Prevotella nigrescens, Streptococcus sanguis, Actinomyces viscosus (i), Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans and Peptostreptococcus micros (i) was evaluated. A 200-μm optical fibre was placed in Eppendorf tubes containing the different bacterial suspensions. At an energy of 170 mJ/30 Hz, 351 pulses of 150 μs were sufficient to kill the most resistant bacteria (i). The exposure time was 28 s. The temperature rise caused by the shots were measured in vitro at the surface of the roots of two groups of 10 single-rooted teeth. One group received 350 pulses; for the other the preparation time was fixed at 28 s. The temperature rose by 25.66°C for the first group and 25.25°C for the second.

In order to set up a disinfection protocol, the tip of the optical fibre of an Nd-YAP laser, λ=1.34 μm, was contaminated in vitro with: Mycobacterium smegmatis (CIP 7326), Candida albicans (ATCC 2091), Bacillus stearothermophilus (ATCC 7953) or Streptococcus sanguis (ATCC 10556). It was then treated with various biocidal solutions, Septinol(®) V, glutaraldehyde 2%, ethanol 95° and NaOCl 6%, using one of two protocols: 10 s of contact, or 5 s of contact followed by 5 s of wiping. The latter technique proved to be the most effective. Whatever the biocidal solution used, all the bacteria adhering to the fibre were eliminated. The protocol was checked in vivo in the canals of 10 teeth presenting root canal infections. No bacteria withstood the treatment with Septinol(®) V. This treatment does not alter the qualities and performance of the fibre as far as light transmission is concerned.

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